Weeks C R, Ferretti J J
Infect Immun. 1986 Apr;52(1):144-50. doi: 10.1128/iai.52.1.144-150.1986.
The gene specifying type A streptococcal exotoxin (speA), also known as erythrogenic toxin, was cloned from the Streptococcus pyogenes bacteriophage T12 genome and analyzed by nucleotide sequencing. The speA gene consists of 753 base pairs and codes for a 29,244-molecular-weight protein. The speA gene product contains a putative 30-amino acid signal peptide, resulting in a molecular weight of 25,787 for the secreted protein. A possible promoter and ribosome-binding site are present in the region upstream from the speA gene, and a transcriptional terminator is located 69 bases downstream from the translational termination codon. The amino acid sequence of the carboxy-terminal portion of the type A streptococcal exotoxin exhibits extensive homology with the carboxy terminus of Staphylococcus aureus enterotoxins B and C1.
编码A群链球菌外毒素（speA，也称为红疹毒素）的基因从化脓性链球菌噬菌体T12基因组中克隆出来，并进行了核苷酸测序分析。speA基因由753个碱基对组成，编码一种分子量为29244的蛋白质。speA基因产物包含一个推定的30个氨基酸的信号肽，因此分泌蛋白的分子量为25787。在speA基因上游区域存在一个可能的启动子和核糖体结合位点，转录终止子位于翻译终止密码子下游69个碱基处。A群链球菌外毒素羧基末端部分的氨基酸序列与金黄色葡萄球菌肠毒素B和C1的羧基末端具有广泛的同源性。
