Forstrom J W, Nelson K A, Nepom G T, Hellström I, Hellström K E
Nature. 1983;303(5918):627-9. doi: 10.1038/303627a0.
Idiotypic networks regulate the immune response to a variety of antigens. Antibodies generated against other antibodies, called anti-idiotypic antibodies, can themselves mimic antigen and elicit a specific immune response. They have been shown to induce delayed-type hypersensitivity (DTH) to model antigens in the mouse. As anti-idiotypic antibodies are thought to be involved in the response to tumour-associated antigens we tested whether injection of monoclonal antibodies derived from mice hyperimmunized to a syngeneic, chemically induced sarcoma could mimic antigen and induce DTH to the sarcoma in naive mice. One of the monoclonal antibodies, 4.72, primed BALB/c mice for DTH to the sarcoma but not for DTH to another sarcoma or to sheep erythrocytes. Antibody 4.72 did not induce DTH in mice of immunoglobulin allotype congeneic strains nor did it bind to the sarcoma cells. As antibodies specific for this sarcoma have not been detected, we do not know whether idiotype on immunoglobulin molecules is recognized by antibody 4.72. However, as the response induced by antibody 4.72 was both antigen-specific and allotype-restricted, analogous to those induced by anti-idiotypic antibodies in other systems, we propose that antibody 4.72 is an anti-idiotypic antibody.
独特型网络调节对多种抗原的免疫反应。针对其他抗体产生的抗体，即抗独特型抗体，自身可模拟抗原并引发特异性免疫反应。已证实它们能在小鼠中诱导对模型抗原的迟发型超敏反应（DTH）。由于抗独特型抗体被认为参与了对肿瘤相关抗原的反应，我们测试了注射源自经同基因化学诱导肉瘤超免疫的小鼠的单克隆抗体是否能模拟抗原并在未免疫小鼠中诱导对该肉瘤的DTH。其中一种单克隆抗体4.72，使BALB/c小鼠对该肉瘤产生DTH致敏，但对另一种肉瘤或绵羊红细胞不产生DTH致敏。抗体4.72在免疫球蛋白同种异型同基因品系的小鼠中不诱导DTH，也不与肉瘤细胞结合。由于尚未检测到针对该肉瘤的特异性抗体，我们不知道免疫球蛋白分子上的独特型是否被抗体4.72识别。然而，由于抗体4.72诱导的反应既是抗原特异性的又是同种异型限制性的，类似于其他系统中抗独特型抗体诱导的反应，我们提出抗体4.72是一种抗独特型抗体。
