Dunn P L, Johnson C A, Styles J M, Pease S S, Dean C J
Immunology. 1987 Feb;60(2):181-6.
Rat X rat hybridomas secreting monoclonal anti-idiotypic antibodies have been prepared from Hooded rats immunized with two tumour-reactive, syngeneic monoclonal antibodies 11/160 and M10/76 (specific, respectively, for the Hooded rat sarcomata HSN and MC24). The hybridomas were selected on the basis that the secreted antibodies competed with antigen for binding to the immunizing idiotype. One monoclonal anti-idiotype (HIM/1/230, gamma 2a isotype) that recognizes an antigen-binding site idiotope of antibody 11/160 has been found to substitute for antigen. Hooded rats vaccinated by three challenges with HIM/1/230 produce serum Ab3 that is indistinguishable in antigen specificity from the 11/160 Ab1, and show reduced tumour take following an i.v. challenge with 10(6) HSN cells. The response to vaccination with anti-idiotype was both qualitatively and quantitatively dependent on the mode of immunization. High titre 11/160-like Ab3 was generated only when the vaccine contained Freund's adjuvant, whereas resistance to tumour challenge was found only in animals vaccinated with anti-idiotype in the absence of adjuvant.
用两种肿瘤反应性同基因单克隆抗体11/160和M10/76(分别对帽状大鼠肉瘤HSN和MC24具有特异性)免疫帽状大鼠后,制备了分泌单克隆抗独特型抗体的大鼠×大鼠杂交瘤。基于分泌的抗体与抗原竞争结合免疫原性独特型来选择杂交瘤。已发现一种识别抗体11/160抗原结合位点独特型的单克隆抗独特型抗体(HIM/1/230,γ2a同种型)可替代抗原。用HIM/1/230进行三次免疫的帽状大鼠产生的血清Ab3在抗原特异性上与11/160 Ab1无法区分,并且在静脉注射10⁶个HSN细胞进行攻击后肿瘤接种率降低。对抗独特型疫苗接种的反应在质量和数量上均取决于免疫方式。仅当疫苗含有弗氏佐剂时才产生高滴度的11/160样Ab3,而仅在无佐剂情况下用抗独特型疫苗接种的动物中发现对肿瘤攻击的抗性。