Nagle R B, Clark V A, McDaniel K M, Davis J R
J Histochem Cytochem. 1983 Aug;31(8):1010-4. doi: 10.1177/31.8.6190854.
A comparison of five immunohistochemical methods for the demonstration of keratins in human ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase--antiperoxidase (PAP), indirect peroxidase, or the avidin--biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.
使用亲和纯化的兔多克隆抗体，对五种免疫组织化学方法在人卵巢肿瘤中检测角蛋白进行了比较。发现在丙酮中短暂固定的冰冻切片上使用间接免疫荧光是最敏感的方法，在所研究的所有14例原发性和1例转移性卵巢上皮性肿瘤中均检测到角蛋白。应用于福尔马林固定组织的蛋白A-过氧化物酶、过氧化物酶-抗过氧化物酶（PAP）、间接过氧化物酶或抗生物素蛋白-生物素化过氧化物酶复合物（ABC）方法敏感性较低，分别在15例中的9例、1例、8例和6例中导致假阴性结果。1例无性细胞瘤用任何方法均未检测到角蛋白。
