Van Voorhis W C, Steinman R M, Hair L S, Luban J, Witmer M D, Koide S, Cohn Z A
J Exp Med. 1983 Jul 1;158(1):126-45. doi: 10.1084/jem.158.1.126.
3C10 and 1D9 are two related monoclonal antibodies that specifically identify human mononuclear phagocytes in a large number of sites, including blood monocytes, alveolar macrophages, and macrophages in tissue sections of spleen, lymph node, and skin. The antigen persists on monocytes cultured for greater than 4 wk, but it is not found on giant cells. The 3C10-1D9 determinant is carried by a 55 kD polypeptide, is expressed at approximately 40,000 copies per monocyte, and is protease sensitive. The antigen is clearly different from HLA-class II or Ia-like antigens that have been studied with a new monoclonal 9.3F10. The 9.3F10 antigen is found on B cells, dendritic cells and monocytes; is protease resistant, and occurs on a 33-29 kD doublet typical of class II products. The 3C10 monoclonal provides a clear distinction between human mononuclear phagocytes and dendritic cells. First, monocytes and lymphocytes can be eliminated from plastic-adherent mononuclear cells using 3C10, complement, and two previously described cytotoxic antibodies, BA-1 (anti-B cell) and Leu-1 (anti-T cell). As a result, the trace dendritic cell component of blood can be enriched to considerable purity (65-75%) and yield. Second, immunocytochemical staining of tissue sections reveals that 3C10+ macrophages are anatomically segregated from dendritic cells. Large numbers of 3C10+ cells are found in red pulp of spleen and in regions surrounding lymphatic channels of lymph node. However, 3C10+ macrophages are scarce in white pulp of spleen and the lymphocyte-rich cortex of node that are the sites where dendritic cells are localized. 3C10+ cells in skin are found in the dermis, particularly in leprosy infiltrates, but the Langerhans' cells of epidermis are 3C10-. The distinctive localization of macrophages and dendritic cells is consistent with their respective functions as effector and accessory cells in the immune response.
3C10和1D9是两种相关的单克隆抗体，它们能在大量部位特异性识别人类单核吞噬细胞，包括血液单核细胞、肺泡巨噬细胞，以及脾脏、淋巴结和皮肤组织切片中的巨噬细胞。该抗原在培养超过4周的单核细胞上持续存在，但在巨细胞上未发现。3C10 - 1D9决定簇由一条55kD的多肽携带，每个单核细胞上约表达40,000个拷贝，且对蛋白酶敏感。该抗原与用新的单克隆抗体9.3F10研究过的HLA - II类或Ia样抗原明显不同。9.3F10抗原存在于B细胞、树突状细胞和单核细胞上；对蛋白酶有抗性，且出现在II类产物典型的33 - 29kD双峰上。3C10单克隆抗体能明确区分人类单核吞噬细胞和树突状细胞。首先，使用3C10、补体以及两种先前描述的细胞毒性抗体BA - 1（抗B细胞）和Leu - 1（抗T细胞），可以从贴壁单核细胞中去除单核细胞和淋巴细胞。结果，血液中微量的树突状细胞成分可被富集到相当高的纯度（65 - 75%）和产量。其次，组织切片的免疫细胞化学染色显示，3C10 +巨噬细胞在解剖学上与树突状细胞分隔开。在脾脏红髓和淋巴结淋巴通道周围区域发现大量3C10 +细胞。然而，在脾脏白髓和淋巴结富含淋巴细胞的皮质（树突状细胞所在部位）中，3C10 +巨噬细胞很少。皮肤中的3C10 +细胞存在于真皮中，特别是在麻风浸润部位，但表皮的朗格汉斯细胞为3C10 -。巨噬细胞和树突状细胞独特的定位与其在免疫反应中作为效应细胞和辅助细胞的各自功能一致。
