Sellon D C, Cullen J M, Whetter L E, Gebhard D H, Coggins L, Fuller F J
Department of Microbiology, Pathology and Parasitology, North Carolina State University College of Veterinary Medicine, Raleigh 27606.
Vet Immunol Immunopathol. 1993 May;36(4):303-18. doi: 10.1016/0165-2427(93)90027-2.
An IgG1 mouse monoclonal antibody, designated 1.646, is described which recognizes a cytoplasmic antigen of equine mononuclear phagocytes. Indirect fluorescent antibody staining of peripheral blood leukocytes reveals a granular cytoplasmic staining, predominantly in adherent blood mononuclear cells. Indirect fluorescent antibody staining is positive for alveolar and peritoneal macrophages. In some horses, a few neutrophils are also stained. In equine tissue samples stained by immunohistochemistry, the distribution of positive cells is consistent with the distribution of tissue macrophages. The most intense and reliable staining occurs with splenic and lymph node macrophages. Hepatic Kupffer cells also stain with antibody 1.646, although the intensity of that staining is somewhat variable between horses. A granular pattern of staining typical of lipofuscin deposition is also seen in liver sections. There is also pale staining of some biliary and renal tubular epithelium. Equine erythrocytes, platelets and lymphocytes are not recognized by this antibody, and neither are monocyte/macrophages of human, canine or feline origin. Antibody 1.646 recognizes two proteins (150 and 30 kDa) of equine monocyte-derived macrophages when assayed by Western immunoblot. Because of the distribution of staining (tissue mononuclear phagocytes, lipofuscin-containing storage granules, biliary and renal tubular epithelium, and some neutrophils) we hypothesize that antibody 1.646 recognizes a cytoplasmic antigen that is closely associated with lysosomal membranes.
描述了一种名为1.646的IgG1小鼠单克隆抗体，它可识别马单核吞噬细胞的一种胞质抗原。外周血白细胞的间接荧光抗体染色显示出颗粒状胞质染色，主要存在于黏附的血液单核细胞中。肺泡巨噬细胞和腹腔巨噬细胞的间接荧光抗体染色呈阳性。在一些马匹中，少数中性粒细胞也被染色。在免疫组织化学染色的马组织样本中，阳性细胞的分布与组织巨噬细胞的分布一致。脾和淋巴结巨噬细胞的染色最为强烈且可靠。肝库普弗细胞也能用抗体1.646染色，不过不同马匹之间该染色强度有所不同。在肝脏切片中还可见到典型的脂褐素沉积的颗粒状染色模式。一些胆管和肾小管上皮也有淡染色。该抗体不能识别马红细胞、血小板和淋巴细胞，也不能识别源自人、犬或猫的单核细胞/巨噬细胞。通过蛋白质免疫印迹法检测时，抗体1.646可识别马单核细胞衍生巨噬细胞的两种蛋白质（150 kDa和30 kDa）。鉴于染色分布情况（组织单核吞噬细胞、含脂褐素的储存颗粒、胆管和肾小管上皮以及一些中性粒细胞），我们推测抗体1.646识别的是一种与溶酶体膜密切相关的胞质抗原。
