Engleman E G, Warnke R, Fox R I, Dilley J, Benike C J, Levy R
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1791-5. doi: 10.1073/pnas.78.3.1791.
A monoclonal antibody (designated L17F12) detects an antigen present on 95-100% of human peripheral T lymphocytes, the majority of thymocytes, and acute lymphocytic leukemia T cells but not B cells, B-cell lines, or monocytes. Examination of frozen tissue sections by the immunoperoxidase method revealed that the cells expressing this antigen were found predominantly in the medulla of thymus and in T-cell zones of lymph node and spleen. The antigen recognized by L17F12 was associated with a cell-surface glycoprotein of 67,000 daltons. L17F12 was used to isolate this molecule from human thymocytes, normal peripheral T cells, leukemic T cells, and T-cell lines. Expression of this antigen on normal T cells was not diminished by prolonged exposure in vitro to various T-cell stimuli. In the absence of complement, L17F12 bound to T cells without altering proliferative functions, thus enabling rapid purification of functionally intact T cells. In the presence of complement, L17F12 was cytolytic for T cells, providing the basis for depletion of T cells from heterogeneous populations. These data suggest that the monoclonal antibody L17F12 recognizes a specific T-cell differentiation protein. This antibody will be useful in studies of the human immune system.
一种单克隆抗体（命名为L17F12）可检测到一种存在于95%至100%的人外周血T淋巴细胞、大多数胸腺细胞以及急性淋巴细胞白血病T细胞上的抗原，但B细胞、B细胞系或单核细胞上则没有该抗原。通过免疫过氧化物酶法对冰冻组织切片进行检查发现，表达这种抗原的细胞主要存在于胸腺髓质以及淋巴结和脾脏的T细胞区。L17F12识别的抗原与一种67000道尔顿的细胞表面糖蛋白相关。L17F12被用于从人胸腺细胞、正常外周血T细胞、白血病T细胞和T细胞系中分离这种分子。正常T细胞上这种抗原的表达不会因在体外长时间暴露于各种T细胞刺激而减少。在没有补体的情况下，L17F12与T细胞结合而不改变其增殖功能，从而能够快速纯化功能完整的T细胞。在有补体存在的情况下，L17F12对T细胞具有细胞溶解作用，为从异质群体中去除T细胞提供了基础。这些数据表明单克隆抗体L17F12识别一种特定的T细胞分化蛋白。这种抗体将有助于人类免疫系统的研究。
