通过用胰蛋白酶肽抑制酶联免疫吸附测定（ELISA）对病毒蛋白抗原决定簇进行定位
Localization of antigenic determinants of a viral protein by inhibition of enzyme-linked immunosorbent assay (ELISA) with tryptic peptides.
作者信息
Altschuh D, Van Regenmortel M H
出版信息
J Immunol Methods. 1982;50(1):99-108. doi: 10.1016/0022-1759(82)90307-6.
PMID:6177792
Abstract
A new method is described for localizing antigenic determinants in globular proteins by means of inhibition of enzyme-linked immunosorbent assay (ELISA) with tryptic peptides. The inhibitory activity of 10 tryptic peptides of the coat protein of 2 strains of tobacco mosaic virus (TMV) was demonstrated by inhibition of ELISA and verified by inhibition of complement fixation tests. These results bring to 7 the number of antigenic determinants of TMV protein that have been defined.
摘要
本文描述了一种通过用胰蛋白酶肽抑制酶联免疫吸附测定（ELISA）来定位球状蛋白中抗原决定簇的新方法。通过ELISA抑制证明了烟草花叶病毒（TMV）2个毒株外壳蛋白的10种胰蛋白酶肽的抑制活性，并通过补体结合试验抑制进行了验证。这些结果使已确定的TMV蛋白抗原决定簇数量增加到7个。
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