Frank E H, Burge B W, Liwnicz B H, Lotspeich L J, White J C, Wechsler S L, Mayfield F H, Keller J T
Exp Cell Res. 1983 Jul;146(2):371-6. doi: 10.1016/0014-4827(83)90138-6.
Cells from cranial and spinal arachnoid membranes of humans were grown in culture. Their growth characteristics, morphology and details of their cytoskeletal composition are described. Arachnoid membranes, obtained at autopsy, were finely minced and incubated in tissue culture medium. Monolayers of cells of homogeneous morphology grew from these tissue fragments. The cells were flat and polygonal. They divided slowly to form nonoverlapping monolayers of low cell density. Electron microscopic examination of cultured arachnoid cells revealed numerous desmosome-like tight junctions and abundant intermediate filaments (tonofilaments). Both morphological features are characteristic of arachnoid cells in situ, but not of cells in the fibroblast-rich dura mater. Immunofluorescence microscopy with monoclonal antibodies demonstrated cytokeratin in the cytoplasm of primary cultures of arachnoid cells. Thus we demonstrated that these cultured cells retained certain of the specific differentiated properties of arachnoid cells in situ and that they are not fibroblasts (which lack tight junctions and cytokeratins). To our knowledge, there have been no previous reports of in vitro growth of arachnoid cells. This in vitro model should be useful in studying the response of arachnoid cells to a variety of substances thought to be involved in the chronic inflammatory condition of the meninges known as arachnoiditis.
对取自人类颅部和脊髓蛛网膜的细胞进行了培养。描述了它们的生长特性、形态以及细胞骨架组成的细节。取自尸检的蛛网膜被精细切碎并在组织培养基中孵育。从这些组织碎片中长出了形态均匀的细胞单层。细胞呈扁平多边形。它们分裂缓慢，形成细胞密度低且不重叠的单层。对培养的蛛网膜细胞进行电子显微镜检查发现了大量桥粒样紧密连接和丰富的中间丝（张力丝）。这两种形态特征都是原位蛛网膜细胞的特征，但不是富含成纤维细胞的硬脑膜中的细胞的特征。用单克隆抗体进行免疫荧光显微镜检查显示蛛网膜细胞原代培养物的细胞质中有细胞角蛋白。因此，我们证明这些培养的细胞保留了原位蛛网膜细胞的某些特定分化特性，并且它们不是成纤维细胞（成纤维细胞缺乏紧密连接和细胞角蛋白）。据我们所知，以前没有关于蛛网膜细胞体外生长的报道。这个体外模型对于研究蛛网膜细胞对各种被认为与称为蛛网膜炎的脑膜慢性炎症状态有关的物质的反应应该是有用的。
