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与急性弓形虫感染相关的抗原特异性免疫球蛋白M抗体的鉴定。

Identification of an antigen-specific immunoglobulin M antibody associated with acute Toxoplasma infection.

作者信息

Erlich H A, Rodgers G, Vaillancourt P, Araujo F G, Remington J S

出版信息

Infect Immun. 1983 Aug;41(2):683-90. doi: 10.1128/iai.41.2.683-690.1983.

DOI:10.1128/iai.41.2.683-690.1983
PMID:6192087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264696/
Abstract

We used the protein gel transfer technique (Western blotting) to analyze the immune response to individual electrophoretically resolved Toxoplasma antigens. Toxoplasma antigens were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The array of resolved proteins was electrophoretically transferred to CNBr-activated filter paper and incubated with serum samples from patients with either acute or chronic Toxoplasma infections or with serum samples from seronegative controls. Serum immunoglobulin G and immunoglobulin M bound to individual Toxoplasma antigens were detected by radioiodinated staphylococcal protein A and a goat anti-human immunoglobulin M antibody, respectively. A complex array of Toxoplasma antigens was revealed by this procedure. Immunoglobulin M directed against a low-molecular-weight antigen was detected in sera from all acutely infected adults (19 sera tested) and was absent from all sera from individuals with chronic infections (9 sera tested) and from sera from seronegative controls (9 sera tested).

摘要

我们使用蛋白质凝胶转移技术（蛋白质印迹法）来分析对经电泳分离的单个弓形虫抗原的免疫反应。弓形虫抗原通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分级分离。分离出的蛋白质阵列通过电泳转移至溴化氰活化滤纸上，并与急性或慢性弓形虫感染患者的血清样本或血清阴性对照的血清样本一起孵育。分别通过放射性碘化葡萄球菌蛋白A和山羊抗人免疫球蛋白M抗体检测与单个弓形虫抗原结合的血清免疫球蛋白G和免疫球蛋白M。通过该程序揭示了一系列复杂的弓形虫抗原。在所有急性感染成年人的血清（检测了19份血清）中检测到针对低分子量抗原的免疫球蛋白M，而在慢性感染个体的所有血清（检测了9份血清）和血清阴性对照的血清（检测了9份血清）中均未检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/b7e7b71b7e65/iai00137-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/b26dc68f3072/iai00137-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/8655b063d176/iai00137-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/4d2f248eaee2/iai00137-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/444949e70eb7/iai00137-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/2342bdf5191e/iai00137-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/b7e7b71b7e65/iai00137-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/b26dc68f3072/iai00137-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/8655b063d176/iai00137-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/4d2f248eaee2/iai00137-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/444949e70eb7/iai00137-0249-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/2342bdf5191e/iai00137-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca08/264696/b7e7b71b7e65/iai00137-0251-a.jpg

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