Duck-Chong C G
J Clin Pathol. 1983 Aug;36(8):910-4. doi: 10.1136/jcp.36.8.910.
In this investigation, the alkaline denaturation of haemoglobin in the blood of pregnant women and in cord blood obtained from newborn infants was followed by measuring the increase in absorbance at 375 nm. As expected, in the absence of detergent, the haemoglobin of cord blood was much more resistant to alkaline denaturation than that of maternal blood. However, in the presence of Triton X-100, a non-ionic detergent, the sensitivity of fetal haemoglobin to alkali was comparable to that of adult haemoglobin. Similar results were obtained using the non-ionic detergents, Brij-35, Tween 80 and Nonidet P40, but the anionic detergent, sodium deoxycholate, was apparently without effect. These findings form the basis of a rapid and sensitive method for discriminating between maternal and fetal blood in biological specimens.
在本研究中，通过测量375nm处吸光度的增加来跟踪孕妇血液和新生儿脐带血中血红蛋白的碱性变性情况。正如预期的那样，在没有去污剂的情况下，脐带血中的血红蛋白比母体血液中的血红蛋白对碱性变性的抵抗力要强得多。然而，在非离子去污剂Triton X-100存在的情况下，胎儿血红蛋白对碱的敏感性与成人血红蛋白相当。使用非离子去污剂Brij-35、吐温80和Nonidet P40也得到了类似的结果，但阴离子去污剂脱氧胆酸钠显然没有效果。这些发现构成了一种快速灵敏的方法的基础，用于区分生物样本中的母体血液和胎儿血液。
