Sundström S, Ny T, Lundgren E
J Interferon Res. 1983;3(2):223-9. doi: 10.1089/jir.1983.3.223.
The early effects of interferon (IFN) on the pattern of protein synthesis in Swiss 3T3 mouse fibroblasts were investigated by two-dimensional gel electrophoresis of 35S-methionine labeled cell extracts. IFN induced within 4 h the synthesis of three proteins with molecular weights of 48, 49, and 50 kD. An increase in the rate of synthesis of a 31 kD protein was also found. These changes were observed after IFN treatment of exponentially growing cells, density-inhibited cells, serum-starved cells, and serum-stimulated quiescent cells. The same protein patterns were obtained from cells treated with IFN-alpha or IFN-beta. The IFN response was blocked by the addition of actinomycin D, implying de novo transcription of the corresponding species of messenger RNA.
通过对用35S-甲硫氨酸标记的细胞提取物进行二维凝胶电泳，研究了干扰素（IFN）对瑞士3T3小鼠成纤维细胞蛋白质合成模式的早期影响。IFN在4小时内诱导了分子量分别为48、49和50 kD的三种蛋白质的合成。还发现一种31 kD蛋白质的合成速率增加。在对指数生长期细胞、密度抑制细胞、血清饥饿细胞和血清刺激的静止细胞进行IFN处理后观察到了这些变化。用IFN-α或IFN-β处理的细胞得到了相同的蛋白质模式。添加放线菌素D可阻断IFN反应，这意味着相应种类的信使RNA进行了从头转录。
