Veomett G E
School of Biological Sciences, University of Nebraska-Lincoln 68588-0118.
J Interferon Res. 1988 Apr;8(2):217-26. doi: 10.1089/jir.1988.8.217.
The interferons (IFNs) have been shown to be antagonistic to the growth stimulatory effects of mitogens on cultured cells. A report of the interactions of IFN-beta and platelet-derived growth factor on BALB/c-3T3 mouse cells established that IFN itself induced the secretion of a limited number of proteins from this cell line. The present work was undertaken to determine if other murine cell lines treated with homologous IFN-beta also secreted new or additional protein(s) in response to this agent and if this response correlated with other phenotypic properties of the cells. The cell lines examined included L929 cells and two derivatives of this line (GM347 and WDIFN), CAK-TK-, Swiss-3T3, and BALB/c-3T3. Each line was exposed to [35S]methionine in the absence and in the presence of IFN-beta, the supernatant fluids collected, and the radioactive, secreted proteins examined by fluorography after electrophoresis through SDS-containing polyacrylamide gels. Two cell lines (GM347 and Swiss-3T3) did not appear to secrete new or additional proteins after IFN treatment. However, four lines (L929, WDIFN, CAK-TK-, and BALB/c-3T3) did secrete new or additional proteins in response to IFN. Thus IFN-induced secretion of protein appeared to be a common but not universal phenomenon. In addition, although the number and apparent size(s) of the IFN-induced, secreted proteins were different in these various lines, one protein (Mr = 89-90,000) appeared to be secreted by each of them. In this respect it was unique. Moreover the IFN-induced secretion of protein did not appear to correlate with the antiviral or antiproliferative effects of IFN.
干扰素（IFNs）已被证明对有丝分裂原对培养细胞的生长刺激作用具有拮抗作用。一份关于干扰素-β与血小板衍生生长因子在BALB/c - 3T3小鼠细胞上相互作用的报告表明，干扰素本身可诱导该细胞系分泌有限数量的蛋白质。本研究旨在确定用同源干扰素-β处理的其他鼠细胞系是否也会因这种因子而分泌新的或额外的蛋白质，以及这种反应是否与细胞的其他表型特性相关。所检测的细胞系包括L929细胞及其两个衍生物（GM347和WDIFN）、CAK - TK -、瑞士3T3细胞和BALB/c - 3T3细胞。每个细胞系在不存在和存在干扰素-β的情况下暴露于[35S]甲硫氨酸，收集上清液，通过含SDS的聚丙烯酰胺凝胶电泳后，用荧光自显影法检测放射性分泌蛋白。两个细胞系（GM347和瑞士3T3）在干扰素处理后似乎没有分泌新的或额外的蛋白质。然而，四个细胞系（L929、WDIFN、CAK - TK -和BALB/c - 3T3）确实因干扰素而分泌了新的或额外的蛋白质。因此，干扰素诱导的蛋白质分泌似乎是一种常见但并非普遍存在的现象。此外，尽管在这些不同的细胞系中，干扰素诱导分泌的蛋白质的数量和表观大小不同，但有一种蛋白质（Mr = 89 - 90,000）似乎在每个细胞系中都会分泌。在这方面它是独特的。而且，干扰素诱导的蛋白质分泌似乎与干扰素的抗病毒或抗增殖作用无关。
