Betina V
Folia Microbiol (Praha). 1976;21(5):362-70. doi: 10.1007/BF02876962.
The mycelium of Trichoderma viride grown in the dark under submerged conditions and transferred to membrane filters sporulated only after photoinduction. The optimum photoinduction of sporulation was reached when applying daylight for 3 min and near ultraviolet radiation (355 nm) for 10 to 30 sec. After the photoinduction probounced synthesis of DNA, RNA and protein was observed. The photoinduced sporulation was partially or fully inhibited in the presence of phenethyl alcohol, actinomycin D, 5-fluorouracil, cycloheximide and ethidium bromide. The same inhibitors blocked also the photoinduced sporulation of surface growing colonies of Trichoderma viride. Various inhibitiors of synthesis of nucleic acids and protein, inhibitors impairing the function of membranes and certain other compounds were also effective.
在黑暗中液体培养条件下生长的绿色木霉的菌丝体，转移到膜滤器上后，只有经过光诱导才会形成孢子。当给予3分钟日光和10至30秒近紫外辐射（355纳米）时，孢子形成的光诱导达到最佳效果。光诱导后观察到DNA、RNA和蛋白质的显著合成。在苯乙醇、放线菌素D、5-氟尿嘧啶、环己酰亚胺和溴化乙锭存在的情况下，光诱导的孢子形成被部分或完全抑制。同样的抑制剂也会阻止绿色木霉表面生长菌落的光诱导孢子形成。各种核酸和蛋白质合成抑制剂、损害膜功能的抑制剂以及某些其他化合物也有效。
