Benìtez T, Villa T G, Acha I G
Arch Microbiol. 1976 Jun;108(2):183-8. doi: 10.1007/BF00428949.
When polyoxin D is added to a spore suspension of Trichoderma viride at a concentration from 50-100 mug/ml, it inhibits from 40-60% of germination. This percentage increases if dimethylsulfoxide (DMSO) is added. Mycelium growing in the presence of polyoxin D becomes irregular and loses its rigidity, showing several bulges along the hypha. Under the electron microscope the features of the cell wall and cytoplasmic content are apparently normal. Nevertheless, after incubation with different lytic systems or with (14C) glucose, it can be seen that polyoxin D partially inhibits the biosynthesis of beta-(1-3) glucan and the biosynthesis of chitin to a greater extent attaining inhibition of 83% at 100 mug/ml of the antibiotic concentration. Rgenerating protoplasts are less affected by polyoxin D. They do regenerate slower but the percentage of regeneration is more than 80%. Aberrant tubes synthesized by these protoplasts are not affected, they manifest their usual morphology and lack of chitin is confirmed in their composition.
当多氧霉素D以50 - 100微克/毫升的浓度添加到绿色木霉的孢子悬浮液中时，它会抑制40 - 60%的孢子萌发。如果添加二甲基亚砜（DMSO），这个百分比会增加。在多氧霉素D存在的情况下生长的菌丝体变得不规则并失去其刚性，在菌丝上出现几个凸起。在电子显微镜下，细胞壁和细胞质内容物的特征显然是正常的。然而，在用不同的裂解系统或与（14C）葡萄糖孵育后，可以看出多氧霉素D部分抑制β-（1-3）葡聚糖的生物合成，并且在100微克/毫升的抗生素浓度下对几丁质生物合成的抑制作用更大，达到83%。再生原生质体受多氧霉素D的影响较小。它们确实再生得较慢，但再生百分比超过80%。由这些原生质体合成的异常管不受影响，它们表现出其通常的形态，并且在其组成中几丁质的缺乏得到证实。
