Johannessen A C, Nilsen R, Skaug N
Scand J Dent Res. 1983 Jun;91(3):191-9. doi: 10.1111/j.1600-0722.1983.tb00801.x.
Thirty-five human dental periapical lesions were studied by immunofluorescence technique using polyclonal anti-IgG (F(ab)2), anti-IgM (F(ab)2), anti-IgA (F(ab)2), anti-IgE and anti-C3c as well as monoclonal anti-IgE. Prewashed ethanol-fixed specimens showed a great number of plasma cells staining for IgG. Cells containing IgA, IgM or IgE were also seen. Deposits of immunoglobulins and C3c suggestive of immune complexes were demonstrated using a double staining technique combining FITC-labeled immunoglobulins with TRITC-conjugated C3c. The complexes were located intracellularly in macrophages, as well as in vessel walls and in the basement membrane zone of proliferating epithelium. Immune complexes may be involved in the epithelial proliferation in those granulomas which lead to cyst formation. Monoclonal anti-IgE demonstrated plasma cells and mast cells, as well as intercellular IgE in epithelial strands of granulomas. The role of IgE in the epithelium is obscure. The study strongly indicates the involvement of hypersensitivity reactions type I and III in periapical inflammatory lesions.
采用多克隆抗IgG（F(ab)2）、抗IgM（F(ab)2）、抗IgA（F(ab)2）、抗IgE和抗C3c以及单克隆抗IgE，通过免疫荧光技术对35例人类牙齿根尖周病变进行了研究。预先洗涤的乙醇固定标本显示大量浆细胞IgG染色阳性。也可见含有IgA、IgM或IgE的细胞。采用将异硫氰酸荧光素（FITC）标记的免疫球蛋白与四甲基异硫氰酸罗丹明（TRITC）偶联的C3c相结合的双重染色技术，证实了提示免疫复合物的免疫球蛋白和C3c沉积。这些复合物位于巨噬细胞内、血管壁以及增殖上皮的基底膜区。免疫复合物可能参与了那些导致囊肿形成的肉芽肿中的上皮增殖。单克隆抗IgE显示了浆细胞、肥大细胞以及肉芽肿上皮条索中的细胞间IgE。IgE在上皮中的作用尚不清楚。该研究有力地表明I型和III型超敏反应参与了根尖周炎症病变。
