Gottstein B, Eckert J, Fey H
Z Parasitenkd. 1983;69(3):347-56. doi: 10.1007/BF00927876.
An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively. By affinity chromatography using rabbit anti hydatid fluid IgG coupled covalently to CNBr-Sepharose 4B a protein fraction (Em 1) containing shared antigens of both parasites could be isolated from an extract of E. multilocularis metacestode tissue. From the same source another antigen fraction (Em 2) with a high degree of specificity for E. multilocularis was prepared by immunosorption. Antigen Em 1 was equally sensitive for the detection of antibodies against E. granulosus and E. multilocularis, whereas antigen fraction Em 2 appeared to be more specific for E. multilocularis. A correct serological differential diagnosis was achieved in 95% of 57 confirmed cases of human cystic or alveolar echinococcosis by the simultaneous use of both antigen fractions in the ELISA and by comparison of their reactivities.
酶联免疫吸附测定（ELISA）被用于对分别由细粒棘球绦虫或多房棘球绦虫引起的人类囊性或泡状棘球蚴病进行血清学鉴别诊断。通过使用共价偶联到溴化氰活化的琼脂糖凝胶4B上的兔抗包虫液IgG进行亲和层析，可从多房棘球绦虫中绦期组织提取物中分离出一种包含两种寄生虫共同抗原的蛋白质组分（Em 1）。通过免疫吸附从同一来源制备了另一种对多房棘球绦虫具有高度特异性的抗原组分（Em 2）。抗原Em 1对检测细粒棘球绦虫和多房棘球绦虫抗体的敏感性相同，而抗原组分Em 2对多房棘球绦虫似乎更具特异性。通过在ELISA中同时使用两种抗原组分并比较它们的反应性，在57例确诊的人类囊性或泡状棘球蚴病病例中，95%实现了正确的血清学鉴别诊断。
