Hussein L, Müller R
Nutr Metab. 1978;22(2):127-40. doi: 10.1159/000176206.
The activity of hepatic lysine-2-oxoglutarate reductase was determined in white female rats maintained on one of two dietary lysine levels (0.23 and 0.54% in diets A and B, respectively). The high dietary lysine increased the enzyme activity (p less than 0.05) twofold compared with the activity obtained with the diet A (67 vs. 27 mumol saccharopine/30 min/g liver protein). Repeated intraperitoneal injection of glucagon (259 microgram/100 g body weight/day) showed a short initial drop in the enzyme activity, followed by a marked rise, which remained above the basal level after withdrawal of the drug. Repeated injections of cycloheximide increased the enzyme activity, and the findings suggested that the apparent increase was due to the inhibition of the system responsible for the degradation of the enzyme. The individual free amino acids of the livers varied as a function of treatment. The ratio of E/N amino acids was found to be a good measure of the physiological condition of the animal, with the lowest value in animals treated with cycloheximide.
在分别维持两种膳食赖氨酸水平（饮食A和饮食B中分别为0.23%和0.54%）的白色雌性大鼠中测定了肝脏赖氨酸-2-氧代戊二酸还原酶的活性。高膳食赖氨酸使酶活性增加了两倍（p<0.05），与饮食A所获得的活性相比（67对27微摩尔saccharopine/30分钟/克肝脏蛋白）。重复腹腔注射胰高血糖素（259微克/100克体重/天）显示酶活性最初有短暂下降，随后显著上升，停药后仍高于基础水平。重复注射环己酰亚胺增加了酶活性，研究结果表明这种明显增加是由于负责酶降解的系统受到抑制。肝脏中各个游离氨基酸随治疗而变化。发现E/N氨基酸比值是衡量动物生理状态的良好指标，在接受环己酰亚胺治疗的动物中该值最低。
