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大鼠肝脏线粒体中L-赖氨酸氧化降解的调控

Regulation of oxidative degradation of L-lysine in rat liver mitochondria.

作者信息

Scislowski P W, Foster A R, Fuller M F

机构信息

Rowett Research Institute, Bucksburn, Aberdeen, U.K.

出版信息

Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):887-91. doi: 10.1042/bj3000887.

DOI:10.1042/bj3000887
PMID:8010974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138248/
Abstract

The generation of 14CO2 from [1-14C]lysine by hepatic mitochondria through the saccharopine pathway is controlled by intramitochondrial concentrations of lysine, 2-oxoglutarate and NADPH. Mitochondria, isolated from rats pre-treated with glucagon, exhibited higher activities of L-lysine: 2-oxoglutarate reductase, saccharopine dehydrogenase and 2-aminoadipate aminotransferase. The flux through this pathway is stimulated in liver mitochondria after glucagon treatment. Multiple regulation of lysine oxidation in liver mitochondria confirms a complex mechanism of 'mitochondrial activation' by glucagon.

摘要

肝脏线粒体通过酵母氨酸途径由[1-¹⁴C]赖氨酸生成¹⁴CO₂的过程受线粒体内赖氨酸、2-氧代戊二酸和NADPH浓度的控制。从用胰高血糖素预处理的大鼠中分离出的线粒体，其L-赖氨酸：2-氧代戊二酸还原酶、酵母氨酸脱氢酶和2-氨基己二酸转氨酶的活性较高。胰高血糖素处理后，肝脏线粒体中通过该途径的通量受到刺激。肝脏线粒体中赖氨酸氧化的多重调节证实了胰高血糖素“线粒体激活”的复杂机制。

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本文引用的文献

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The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. I. Activities at different pH values.肝脏线粒体对三磷酸腺苷的酶促水解作用。I. 不同pH值下的活性
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