Coon J, Hunter R
J Immunol. 1975 May;114(5):1518-22.
If bovine serum albumin (BSA) is covalently conjugated with dodecanoic acid, its ability to stimulate delayed-type hypersensitivity (DTH) is enhanced whereas its ability to stimulate antibody production is suppressed. Conjugates of BSA prepared with negatively charged succinyl groups and positively charged amidine groups were unable to stimulate any detectable DTH to BSA even though their ability to bind anti-BSA antibody in a radioimmunoassay was similar to that of dodecanoyl-BSA which stimulated strong, sustained DTH. By analyzing a series of conjugates of BSA with fatty acids of varying chain length, we found that enhancement of the ability of a preparation to stimulate DTH to BSA could be separated from the suppression of its ability to stimulate antibody formation. The ability of a preparation to stimulate DTH correlated with the covalent conjugation of a large number of hydrophobic groups whereas the suppression of the ability to stimulate antibody formation correlated with covering of antibody-combining sites as measured in a radioimmunoassay.
如果将牛血清白蛋白（BSA）与十二烷酸共价结合，其刺激迟发型超敏反应（DTH）的能力会增强，而刺激抗体产生的能力则会受到抑制。用带负电荷的琥珀酰基团和带正电荷的脒基团制备的BSA缀合物，即使它们在放射免疫测定中结合抗BSA抗体的能力与能刺激强烈、持续DTH的十二烷酰-BSA相似，也无法刺激对BSA产生任何可检测到的DTH。通过分析一系列BSA与不同链长脂肪酸的缀合物，我们发现制剂刺激对BSA的DTH能力的增强可以与其刺激抗体形成能力的抑制相分离。制剂刺激DTH的能力与大量疏水基团的共价结合相关，而刺激抗体形成能力的抑制与放射免疫测定中所测量的抗体结合位点的覆盖相关。
