炎症期间四种急性期蛋白的信使核糖核酸活性。
Messenger RNA activities of four acute phase proteins during inflammation.
作者信息
Northemann W, Andus T, Gross V, Nagashima M, Schreiber G, Heinrich P C
出版信息
FEBS Lett. 1983 Sep 19;161(2):319-22. doi: 10.1016/0014-5793(83)81033-3.
Poly(A)+ RNA isolated from the livers of normal rats and of rats suffering from an acute inflammation was translated in a cell-free translation system from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against alpha 1-acid glycoprotein, alpha 2-macroglobulin, transferrin, alpha 1-proteinase inhibitor and albumin. 15 to 21 h after intramuscular injection of turpentine 73-, 66-, 2.8-, and 2-fold increases in translatable mRNAs for alpha 1-acid glycoprotein, alpha 2-macroglobulin, transferrin and alpha 1-proteinase inhibitor, respectively, were observed. For albumin a decrease in translatable mRNA to about 30% of controls was measured.
从正常大鼠和患有急性炎症的大鼠肝脏中分离出的多聚腺苷酸（Poly(A)）+ RNA，在兔网织红细胞的无细胞翻译系统中进行翻译。翻译产物用针对α1-酸性糖蛋白、α2-巨球蛋白、转铁蛋白、α1-蛋白酶抑制剂和白蛋白的特异性抗血清进行免疫沉淀。肌肉注射松节油15至21小时后，分别观察到α1-酸性糖蛋白、α2-巨球蛋白、转铁蛋白和α1-蛋白酶抑制剂的可翻译mRNA增加了73倍、66倍、2.8倍和2倍。对于白蛋白，可翻译mRNA减少至对照的约30%。
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