大鼠肺组织是α1-蛋白酶抑制剂的合成部位：无细胞翻译实验证据。

Rat lung tissue is a site of alpha 1-proteinase inhibitor synthesis: evidence by cell-free translation.

作者信息

Budek W, Bünning P, Heinrich P C

出版信息

Biochem Biophys Res Commun. 1984 Jul 18;122(1):394-400. doi: 10.1016/0006-291x(84)90488-1.

Abstract

Poly(A) +RNA isolated from lungs of normal rats and of rats suffering from experimental inflammation was translated in a cell-free translation mixture from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against alpha 1-proteinase inhibitor and alpha 2-macroglobulin. Comparable levels of mRNA for alpha 1-proteinase inhibitor were found in rat lung tissue from control and experimentally inflamed animals. alpha 2-Macroglobulin mRNA could not be detected in rat lung tissue.

摘要

从正常大鼠和实验性炎症大鼠的肺中分离出的多聚腺苷酸（Poly(A)）+RNA，在兔网织红细胞的无细胞翻译混合物中进行翻译。翻译产物用针对α1-蛋白酶抑制剂和α2-巨球蛋白的特异性抗血清进行免疫沉淀。在对照动物和实验性炎症动物的大鼠肺组织中，发现α1-蛋白酶抑制剂的mRNA水平相当。在大鼠肺组织中未检测到α2-巨球蛋白mRNA。