Watson S J, Khachaturian H, Seidah N G, Chretien M, Zimmerman E, Nilaver G, van Wimersma Gredianus T B
Neuropeptides. 1983 Jul;3(5):321-36. doi: 10.1016/0143-4179(83)90021-5.
Arginine-vasopressin (AVP) and its associated neurophysin (AVP-NP) are synthesized via a precursor, propressophysin, which also contains a 39 amino acid glycopeptide at its C-terminus (C-terminus of propressophysin, or CPP). In the present study, immunocytochemical techniques were used to determine the cellular co-localization of CPP with AVP, oxytocin (OXY), AVP-NP and OXY-NP in the rat hypothalamus using colchicine pre-treatment and serial 5 micron section analysis. Extensive cross-competition studies of antisera raised against each peptide with the various antigens yielded no significant crossreactivity of the CPP, AVP, OXY and NP antisera. The NP antiserum, although directed against both AVP-NP and OXY-NP, demonstrated a preference for OXY-NP at a dilution of 1:20,000. CPP and AVP were always co-localized within the same magnocellular neurons of the supraoptic, paraventricular and circularis nuclei, and further showed very similar patterning in the suprachiasmatic nucleus as well. In contrast, no cellular overlap could be detected between CPP and OXY, in any of the above nuclei (the suprachiasmatic nucleus is devoid of OXY). Likewise, no examples of co-localization of CPP and OXY-NP were found in the magnocellular nuclei. These results are in strong agreement with a biosynthetic relationship between CPP, AVP and AVP-NP, and their separateness from the OXY and OXY-NP precursor.
精氨酸加压素（AVP）及其相关的神经垂体素（AVP-NP）是通过一种前体——加压素原合成的，加压素原在其C末端还含有一个39个氨基酸的糖肽（加压素原的C末端，或CPP）。在本研究中，采用免疫细胞化学技术，通过秋水仙碱预处理和连续5微米切片分析，来确定CPP与AVP、催产素（OXY）、AVP-NP和OXY-NP在大鼠下丘脑的细胞共定位情况。用针对每种肽产生的抗血清与各种抗原进行广泛的交叉竞争研究，结果显示CPP、AVP、OXY和NP抗血清之间没有明显的交叉反应性。NP抗血清虽然针对AVP-NP和OXY-NP，但在1:20,000的稀释度下对OXY-NP表现出偏好。CPP和AVP总是共定位于视上核、室旁核和环状核的同一大细胞神经元内，并且在视交叉上核中也显示出非常相似的模式。相比之下，在上述任何核中（视交叉上核不含OXY），均未检测到CPP和OXY之间的细胞重叠。同样，在大细胞核中也未发现CPP和OXY-NP共定位的例子。这些结果与CPP、AVP和AVP-NP之间的生物合成关系以及它们与OXY和OXY-NP前体的分离情况高度一致。
