König A L, Kather H, Roelcke D
Blut. 1984 Nov;49(5):363-8. doi: 10.1007/BF00319883.
In association with atypical pneumonia, a patient developed acute severe autoimmune hemolytic anemia. Hemoglobin temporarily was only 7.0 g/100 ml, so that the patient needed red blood cell (RBC) transfusion. Hemolysis was found to be caused by high titer cold agglutinins (CA), which occurred transiently during the acute period of the disease. CA of two different specificities, anti-I and anti-Fl, were demonstrated in the patient's serum. Antibodies of the two specificities were clearly separated by absorption/elution experiments using neuraminidase (RDE)-treated RBC. They were distinguished by serologic means: Both anti-I and anti-Fl react more strongly with adult RBC than with newborn and i adult RBC; in contrast to anti-I, anti-Fl does not agglutinate RDE-treated cells. Inhibition experiments showed that I-active substances prepared from papainized RBC exhibited both I and Fl antigenic activity. By RDE-treatment of I-active substances, Fl-activity was markedly reduced, while I-activity was increased.
一名患者在患非典型肺炎的同时，并发了急性重症自身免疫性溶血性贫血。血红蛋白一度仅为7.0 g/100 ml，因此患者需要输注红细胞。溶血是由高滴度冷凝集素（CA）引起的，在疾病急性期短暂出现。在患者血清中检测到两种不同特异性的CA，即抗-I和抗-Fl。通过使用神经氨酸酶（RDE）处理的红细胞进行吸收/洗脱实验，可清晰分离出这两种特异性抗体。它们可通过血清学方法进行区分：抗-I和抗-Fl与成人红细胞的反应均强于新生儿红细胞和i成人红细胞；与抗-I不同，抗-Fl不会凝集经RDE处理的细胞。抑制实验表明，由木瓜蛋白酶处理的红细胞制备的I活性物质同时具有I和Fl抗原活性。通过对I活性物质进行RDE处理，Fl活性显著降低，而I活性增加。
