Johnson D A, Elder J H
J Exp Med. 1983 Nov 1;158(5):1751-6. doi: 10.1084/jem.158.5.1751.
We prepared an antiserum to a 14-amino-acid-long synthetic peptide derived from the published nucleotide sequence of a Moloney virus-derived MCF recombinant virus. This peptide is not found in the parental Moloney virus gp70 sequence or in other ecotropic viruses so far sequenced. When several tissues of the mouse were examined by Western blot analysis for related molecules, we found that thymocytes from 2-3-mo-old mice, but not splenic T cells or bone marrow cell extracts reacted with this probe. We found that the Mr 70,000 thymic glycoprotein recognized by the anti-peptide antiserum (81-TAg) was present at equivalent levels on peanut agglutinin positive thymocytes in all mouse strains tested. These results indicate that 81-TAg is a differentiation antigen that is related to the substituted portion of an MCF retrovirus.
我们制备了一种抗血清,用于针对一种由莫洛尼病毒衍生的MCF重组病毒已发表的核苷酸序列推导而来的14个氨基酸长的合成肽。该肽在亲本莫洛尼病毒gp70序列或迄今测序的其他亲嗜性病毒中均未发现。当通过蛋白质免疫印迹分析检测小鼠的多个组织以寻找相关分子时,我们发现2至3月龄小鼠的胸腺细胞与该探针发生反应,而脾T细胞或骨髓细胞提取物则无此反应。我们发现,抗肽抗血清(81-TAg)识别的分子量为70,000的胸腺糖蛋白在所有测试小鼠品系的花生凝集素阳性胸腺细胞上以等量水平存在。这些结果表明,81-TAg是一种与MCF逆转录病毒的取代部分相关的分化抗原。