Bosselman R A, van Straaten F, Van Beveren C, Verma I M, Vogt M
J Virol. 1982 Oct;44(1):19-31. doi: 10.1128/JVI.44.1.19-31.1982.
A biologically active molecular clone of BALB/Moloney mink cell focus-forming (Mo-MCF) proviral DNA has been reconstructed in vitro. It contains the 5' half of BALB/Moloney murine leukemia virus (Mo-MuLV) DNA and the 3' half of BALB/Mo-MCF DNA. The complete nucleotide sequence of the env gene and the 3' long terminal repeat (LTR) of the cloned Mo-MCF DNA has been determined and compared with the sequence of the corresponding region of parental Mo-MuLV DNA. The substitution in the Mo-MCF DNA encompasses 1,159 base pairs, beginning in the carboxyl terminus of the pol gene and extending to the middle of the env gene. The Mo-MCF env gene product is predicted to be 29 amino acids shorter than the parental Mo-MuLV env gene product. The portion of the env gene encoding the p15E peptide is identical in both viral DNAs. There is an additional A residue in the Mo-MCF viral DNA in a region just preceding the 3' LTR. The nucleotide sequence of the 3' LTR of Mo-MCF DNA is similar to that of the 5' LTR of BALB/Mo-MuLV DNA with the exception of two single base substitutions. We conclude that the sequence substitution in the env gene is responsible for the dual-tropic properties of Mo-MCF viruses.
一种具有生物活性的BALB/莫洛尼貂细胞集落形成（Mo-MCF）前病毒DNA分子克隆已在体外重建。它包含BALB/莫洛尼鼠白血病病毒（Mo-MuLV）DNA的5' 端一半和BALB/Mo-MCF DNA的3' 端一半。已确定克隆的Mo-MCF DNA的env基因和3' 长末端重复序列（LTR）的完整核苷酸序列，并与亲本Mo-MuLV DNA相应区域的序列进行了比较。Mo-MCF DNA中的取代涵盖1159个碱基对，从pol基因的羧基末端开始，延伸至env基因的中部。预计Mo-MCF env基因产物比亲本Mo-MuLV env基因产物短29个氨基酸。两种病毒DNA中编码p15E肽的env基因部分是相同的。在Mo-MCF病毒DNA中，3' LTR之前的一个区域有一个额外的A残基。Mo-MCF DNA的3' LTR核苷酸序列与BALB/Mo-MuLV DNA的5' LTR相似，只是有两个单碱基取代。我们得出结论，env基因中的序列取代是Mo-MCF病毒双嗜性特性的原因。
