Barta A, Kuechler E
FEBS Lett. 1983 Nov 14;163(2):319-23. doi: 10.1016/0014-5793(83)80844-8.
Upon irradiation, 3-[4-benzoylphenyl]propionyl-PhetRNA bound to the P-site of poly(U)-primed ribosomes is exclusively cross-linked to 23S RNA. It is shown that the photoreaction only occurs with pyrimidine nucleotides. The site of the cross-link is located within an 11S RNA fragment, which comprises the 1100 nucleotides at the 3'-end of 23S RNA. The cross-linked Phe tRNA derivative is still functionally active in peptide bond formation. The site labelled on the 11S fragment is therefore an integral part of the peptidyltransferase centre.
经照射后，与聚（U）引发的核糖体P位点结合的3-[4-苯甲酰基苯基]丙酰-Phe tRNA仅与23S RNA发生交联。结果表明，光反应仅发生在嘧啶核苷酸上。交联位点位于一个11S RNA片段内，该片段包含23S RNA 3'端的1100个核苷酸。交联的苯丙氨酸tRNA衍生物在肽键形成中仍具有功能活性。因此，11S片段上标记的位点是肽基转移酶中心的一个组成部分。
