Minagawa H, Shiosaka S, Inoue H, Hayashi N, Kasahara A, Kamata T, Tohyama M, Shiotani Y
Gastroenterology. 1984 Jan;86(1):51-9.
The origin and three-dimensional distribution of substance P-immunoreactive structures in the rat stomach were investigated by means of the indirect immunofluorescence method using whole-mount tissue. The present study demonstrated that the myenteric plexus contained a very high concentration of substance P-immunoreactive fibers. It also contained cells that were moderately concentrated in the greater curvature, with those closer to the lesser curvature decreasing in number. In addition, a number of fibers ran parallel to the muscles in the circular and longitudinal muscle layers; these were distributed evenly throughout the entire stomach. We further found that most of the fibers located in the myenteric plexus originated from the greater splanchnic nerve from T5 to L2, but that some originated from intrinsic substance P-immunoreactive cells in the myenteric plexus. The fibers in the circular and longitudinal muscle layers originated from the cells in the myenteric plexus.
采用整装组织间接免疫荧光法，研究了大鼠胃中P物质免疫反应性结构的起源和三维分布。本研究表明，肌间神经丛含有非常高浓度的P物质免疫反应性纤维。它还含有一些细胞，这些细胞在胃大弯处浓度适中，靠近胃小弯处的细胞数量逐渐减少。此外，许多纤维与环行肌层和纵行肌层中的肌肉平行排列；它们均匀地分布于整个胃。我们进一步发现，位于肌间神经丛的大多数纤维起源于T5至L2的内脏大神经，但也有一些起源于肌间神经丛中内源性P物质免疫反应性细胞。环行肌层和纵行肌层中的纤维起源于肌间神经丛中的细胞。
