Zak K P, Filatova R S, Afanasyeva V V
Folia Haematol Int Mag Klin Morphol Blutforsch. 1983;110(4):490-502.
Cytochemically peroxidase activity has been examined on the light optical and ultrastructural levels in blood granulocytes of the rabbits after a single (5 mg/kg) and multiple (1 and 5 mg/kg every 24 hrs during 4 weeks) administrations of hydrocortisone. Under electron microscope peroxidase activity was detected in the blood of intact rabbits into typical primary granules (TPG) and small polymorphic granules (SPG) of neutrophils as well as into specific granules of basophils sometimes in perinuclear space and GER channels. 6 h after hydrocortisone injection peroxidase activity in neutrophils increased, the reaction product in both kinds of cytoplasmic granules was electron denser than in the controls. After multiple hydrocortisone (1 mg/kg) administrations peroxidase general activity in granulocytes has not considerably changed, but the number of TPGs and SPGs was decreased in neutrophils. Multiple administrations of a higher dose of hydrocortisone (5 mg/kg) have induced peroxidase activity decreasing in neutrophils and a decrease in the number and electron density of TPGs and SPGs in them. In basophils there was a significant accumulation of the reaction product of high electron density in perinuclear space, in specific granules and GER channels. The conclusions has been drawn that a short-term raising of hydrocortisone level stimulates and prolonged hypercorticism inhibits peroxidase activity in neutrophils and, consequently, their function.
在单次（5毫克/千克）和多次（4周内每24小时1毫克/千克和5毫克/千克）给予氢化可的松后，对兔血液粒细胞进行了光镜和超微结构水平的细胞化学过氧化物酶活性检测。在电子显微镜下，完整兔血液中的过氧化物酶活性在中性粒细胞的典型初级颗粒（TPG）和小多形性颗粒（SPG）以及嗜碱性粒细胞的特异性颗粒中被检测到，有时在核周间隙和GER通道中也能检测到。氢化可的松注射6小时后，中性粒细胞中的过氧化物酶活性增加，两种细胞质颗粒中的反应产物电子密度均高于对照组。多次给予氢化可的松（1毫克/千克）后，粒细胞中的过氧化物酶总体活性没有明显变化，但中性粒细胞中的TPG和SPG数量减少。多次给予较高剂量的氢化可的松（5毫克/千克）导致中性粒细胞过氧化物酶活性降低，其中TPG和SPG的数量及电子密度均下降。在嗜碱性粒细胞中，核周间隙、特异性颗粒和GER通道中有高电子密度的反应产物显著积聚。得出的结论是，氢化可的松水平的短期升高刺激，而长期皮质醇增多症抑制中性粒细胞中的过氧化物酶活性，从而抑制其功能。
