Portela-Gomes G M, Aguas J M, Matos A P, Grimelius L
Department of Medicine II, University Hospital of Santa Maria, Lisbon, Portugal.
Histochem J. 1987 Aug;19(8):449-53. doi: 10.1007/BF01675756.
On the basis of staining results in closely related semi-thin sections from rat antral mucosa immunostained with polyclonal serotonin antibodies and silver-stained for the argentaffin reaction, respectively, three different cell populations could be distinguished. One of these cell populations showed both serotonin immunoreactivity and an argentaffin reaction, a second one serotonin immunoreactivity alone, and a third one only an argentaffin reaction. These cell populations were studied electron microscopically in ultra-thin sections located between the stained semi-thin sections. The cell population displaying an agentaffin reaction and serotonin immunoreactivity showed secretory granules of the enterochromaffin cell type. A similar granular appearance was observed in cells which only exhibited an argentaffin reaction. Serotonin immunoreactivity in the absence of an argentaffin reaction was evident in some G (gastrin) cells, and in some D1 and possibly also some D (somatostatin) cells; but not all the endocrine cells of the non-enterochromaffin type displayed serotonin immunoreactivity. The significance of the different reactions in the three cell populations is discussed.
根据用多克隆血清素抗体免疫染色和分别进行嗜银反应银染色的大鼠胃窦黏膜密切相关半薄切片的染色结果，可以区分出三种不同的细胞群。其中一个细胞群既显示血清素免疫反应性又显示嗜银反应，第二个仅显示血清素免疫反应性，第三个仅显示嗜银反应。在位于染色半薄切片之间的超薄切片中对这些细胞群进行了电子显微镜研究。显示嗜银反应和血清素免疫反应性的细胞群呈现肠嗜铬细胞类型的分泌颗粒。在仅表现出嗜银反应的细胞中也观察到类似的颗粒外观。在一些G（胃泌素）细胞以及一些D1细胞和可能还有一些D（生长抑素）细胞中，明显存在无嗜银反应的血清素免疫反应性；但并非所有非肠嗜铬细胞类型的内分泌细胞都显示血清素免疫反应性。文中讨论了这三种细胞群中不同反应的意义。
