枯草芽孢杆菌的肽聚糖周转速率与细胞生长速率相关。
Rates of peptidoglycan turnover and cell growth of Bacillus subtilis are correlated.
作者信息
Cheung H Y, Vitković L, Freese E
出版信息
J Bacteriol. 1983 Dec;156(3):1099-106. doi: 10.1128/jb.156.3.1099-1106.1983.
Abstract
Peptidoglycan turnover was measured by the decrease of trichloroacetic acid-precipitable label in cells labeled with N-acetyl-D-[14C]glucosamine. The rate of turnover was reduced strongly by the inhibition of RNA or protein synthesis and weakly by the inhibition of lipid, peptidoglycan, or DNA synthesis. It increased with the growth rate (which was controlled by the concentration of oxomethylvalerate limiting the intracellular isoleucine supply) to the same degree in stringent (rel+) and isogenic relaxed (relA) strains. In these and all other strains tested, the turnover rate (k) increased with the growth rate (g) according to the equation, k = 0.70 X g1.38, even when the growth rate was systematically altered by changes in the temperature or in the composition of the medium.
摘要
用N-乙酰-D-[¹⁴C]葡萄糖胺标记细胞，通过三氯乙酸可沉淀标记物的减少来测定肽聚糖周转。RNA或蛋白质合成的抑制会强烈降低周转速率，而脂质、肽聚糖或DNA合成的抑制则会轻微降低周转速率。在严格（rel⁺）和同基因松弛（relA）菌株中，周转速率随生长速率（由限制细胞内异亮氨酸供应的氧甲基戊酸浓度控制）增加的程度相同。在这些以及所有其他测试菌株中，即使生长速率通过温度或培养基组成的变化而系统性改变，周转速率（k）仍根据方程k = 0.70×g¹·³⁸随生长速率（g）增加。
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