Stanisz A M, Lieberman R, Kaplan A, Davie J M
Mol Immunol. 1983 Sep;20(9):983-8. doi: 10.1016/0161-5890(83)90039-1.
There are two different forms of mouse IgA immunoglobulins, one in which light chains are disulfide-linked to each other (IgAL-L) and the second where light chains are linked to heavy chains (IgAH-L). IgA myeloma proteins from BALB/c mice are IgAL-L and those from NZB are IgAH-L. To determine whether these two forms of IgA represent allotypic or isotypic variants, we purified serum IgA and prepared IgA-secreting somatic cell hybrids from mitogen-stimulated BALB/c and NZB mice and determined the pattern of light chain linkage. It was found that each strain produces both forms of IgA and that both forms share the allotypic determinants characteristic of the mouse strain.
小鼠IgA免疫球蛋白有两种不同形式，一种是轻链通过二硫键相互连接（IgAL-L），另一种是轻链与重链相连（IgAH-L）。来自BALB/c小鼠的IgA骨髓瘤蛋白是IgAL-L，而来自NZB小鼠的是IgAH-L。为了确定这两种形式的IgA是同种异型还是同种型变体，我们纯化了血清IgA，并从丝裂原刺激的BALB/c和NZB小鼠制备了分泌IgA的体细胞杂种，然后确定轻链连接模式。结果发现，每个品系都产生这两种形式的IgA，并且这两种形式都具有该小鼠品系特有的同种异型决定簇。
