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荧光显微镜下直接涂片抗酸杆菌机器染色与手工染色的比较。

Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy.

作者信息

Clancey J K, Allen B W, Rogers D T, Smith L S, Aber V, Mitchison D A

出版信息

J Clin Pathol. 1976 Oct;29(10):931-3. doi: 10.1136/jcp.29.10.931.

Abstract

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears prepared with the machine.

摘要

在卢萨卡和伦敦,对痰液及其他类型标本的直接涂片采用金胺酚进行手工染色和自动机器染色,以检测抗酸杆菌。未发现机器染色过程中抗酸杆菌从阳性涂片转移至阴性涂片的情况。机器制备的涂片上,杆菌与背景之间的对比度有所提高。

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