Naessens J, Sileghem M, MacHugh N, Park Y H, Davis W C, Toye P
ILRAD, Nairobi, Kenya.
Immunology. 1992 Jun;76(2):305-9.
The bovine interleukin-2 receptor-alpha (IL-2R alpha) gene has been isolated and a rabbit antiserum against a fusion protein of the gene has been produced. However, the antiserum does not inhibit IL-2-dependent proliferation. Since a panel of monoclonal antibodies (mAb) to bovine activation antigens was available, we transfected the gene into mouse L fibroblasts, selected stable transfectants with the rabbit antiserum, and screened for antibodies that bound the transfected cells but not the untransfected cells. Three mAb were selected and all three precipitated a molecule of M(r) 55,000 (under reducing conditions) from activated cells, as expected from homology with mouse and human IL-2R alpha (CD25, Tac). One of the three mAb was a strong inhibitor of IL-2-dependent proliferation of bovine lymphocytes. Thus, the availability of transfected cells allowed us to establish quickly and unequivocally the antigenic specificity of a number of antibodies.
牛白细胞介素-2受体α（IL-2Rα）基因已被分离出来，并制备了针对该基因融合蛋白的兔抗血清。然而，该抗血清并不抑制IL-2依赖的增殖。由于有一组针对牛活化抗原的单克隆抗体（mAb），我们将该基因转染到小鼠L成纤维细胞中，用兔抗血清筛选出稳定的转染子，并筛选能与转染细胞而非未转染细胞结合的抗体。挑选出三种单克隆抗体，正如与小鼠和人IL-2Rα（CD25，Tac）的同源性所预期的那样，这三种抗体在还原条件下均从活化细胞中沉淀出一个分子量为55,000的分子。三种单克隆抗体中的一种是牛淋巴细胞IL-2依赖增殖的强抑制剂。因此，转染细胞的可用性使我们能够快速且明确地确定多种抗体的抗原特异性。
