Sharrow S O, Mathieson B J, Singer A
J Immunol. 1981 Apr;126(4):1327-35.
The phenotypic appearance of cell surface antigens on murine thymocytes from long-term radiation bone marrow chimeras was analyzed using indirect immunofluorescence and flow microfluorometry. Cells maturing in the thymi of these mice were typed for MHC (Kk, I-Ak, H-2b, Kb, and Ib) and non-MHC (Lyt 1, Ly 9, and TL) determinants. All cells were of donor origin as determined by non-MHC (Ly) phenotype in P1 leads to P2, P1 X P2 leads to P1, and P1 leads to P2 radiation chimeras. In contrast, the MHC phenotypes of these thymocytes were markedly affected by the host environment. Specifically, H-2 and I-A determinants of both parental phenotypes were detected on thymocytes from P1 leads to P1 X P2 chimeras; I-A determinants of host phenotype were present, whereas I-A determinants of donor phenotype were reduced on thymocytes from P1 X P2 leads to P1 chimeras; and thymocytes from P1 leads to P2 chimeras possessed H-2 and I-A determinants of host phenotype but showed reduction of donor I-A phenotype determinants. The appearance of host cell surface H-2 and I-A determinants on thymocytes from chimeras closely parallels the functional recognition of MHC determinants by T cells from chimeric mice and thus may be significantly related to the development of the self-recognition repertoire by maturing T cells.
利用间接免疫荧光法和流式微量荧光测定法，分析了长期辐射骨髓嵌合体小鼠胸腺细胞表面抗原的表型。对这些小鼠胸腺中成熟的细胞进行了MHC（Kk、I-Ak、H-2b、Kb和Ib）和非MHC（Lyt 1、Ly 9和TL）决定簇的分型。在P1到P2、P1×P2到P1以及P1到P2辐射嵌合体中，通过非MHC（Ly）表型确定所有细胞均来自供体。相反，这些胸腺细胞的MHC表型受到宿主环境的显著影响。具体而言，在P1到P1×P2嵌合体的胸腺细胞上检测到了双亲表型的H-2和I-A决定簇；宿主表型的I-A决定簇存在，而供体表型的I-A决定簇在P1×P2到P1嵌合体的胸腺细胞上减少；P1到P2嵌合体的胸腺细胞具有宿主表型的H-2和I-A决定簇，但供体I-A表型决定簇减少。嵌合体胸腺细胞上宿主细胞表面H-2和I-A决定簇的出现与嵌合体小鼠T细胞对MHC决定簇的功能识别密切平行，因此可能与成熟T细胞自我识别库的发育显著相关。
