Tosi R, Tanigaki N, Sorrentino R, Centis D, Ferrara G B
J Immunol. 1984 Jan;132(1):277-82.
Two 8th Workshop alloantisera, 8W1062 and 8W614, which were classified respectively as anti-DR5 and anti-DR3, were found to react with an Ia preparation from LG38 cells (DR5,5) that was depleted of DR and BR molecules and enriched in DC molecules by pretreatment with a monoclonal antibody and an antiserum against alpha-subunits of DR and BR molecules. The reaction of both alloantisera was inhibited by DR3-positive, DR5-positive, and DRW13-positive individuals. This pattern does not correspond to any of the hitherto reported supertypic specificities. Both antisera were shown to bind to the alpha-subunit isolated from the DC-enriched preparation but not to the beta-subunit. The specificity has been named DC alpha 3. Family studies show the segregation according to the HLA haplotype patterns. This demonstrates by formal genetics criteria the HLA linkage of the locus controlling the DC alpha-subunit.
两种第八届研讨会同种异体抗血清，8W1062和8W614，分别被归类为抗DR5和抗DR3，被发现可与来自LG38细胞（DR5,5）的Ia制剂发生反应，该制剂通过用针对DR和BR分子α亚基的单克隆抗体和抗血清进行预处理，去除了DR和BR分子并富集了DC分子。两种同种异体抗血清的反应均受到DR3阳性、DR5阳性和DRW13阳性个体的抑制。这种模式与迄今报道的任何超型特异性均不相符。两种抗血清均显示与从富含DC的制剂中分离出的α亚基结合，但不与β亚基结合。这种特异性被命名为DCα3。家系研究表明其根据HLA单倍型模式进行分离。这通过正式的遗传学标准证明了控制DCα亚基的基因座与HLA的连锁关系。
