Chambers W H, Klesius P H
Vet Immunol Immunopathol. 1983 Nov;5(1):85-95. doi: 10.1016/0165-2427(83)90034-x.
A direct leukocyte migration inhibition (LMI) assay under agarose was standardized for use in cattle. The procedure included maintenance of culture conditions at pH 7.4 in a humidified, 39 C incubator with 98% air and 2% CO2. Optimum demonstration of incubation was obtained using a 9-hour incubation period. The LMI assay was used to detect reactivity to keyhold limpet hemocyanin (KLH) and horseshoe crab hemocyanin (HCH). Calves sensitized to KLH had specific reactivity for KLH and occasional reactivity for HCH. Similarly, calves sensitized to HCH showed reactivity for HCH and occasional reactivity for KLH. The nonspecific reactivity in the LMI was more frequent after skin testing with both antigens. Specific association of LMI reactivity and DTH reactivity was found in 8 of 10 animals. There were no nonspecific skin test responses. The results suggest that consecutive LMI assays are required to establish trends in reactivity and these should be used in conjunction with other in vitro assays of cellular immune reactivity.
一种用于牛的琼脂糖下直接白细胞迁移抑制（LMI）试验被标准化。该程序包括在湿度为98%空气和2%二氧化碳的39℃加湿培养箱中，将培养条件维持在pH 7.4。使用9小时的孵育期可获得最佳的孵育效果。LMI试验用于检测对钥孔血蓝蛋白（KLH）和鲎血蓝蛋白（HCH）的反应性。对KLH致敏的小牛对KLH有特异性反应，偶尔对HCH有反应。同样，对HCH致敏的小牛对HCH有反应，偶尔对KLH有反应。在用两种抗原进行皮肤试验后，LMI中的非特异性反应更频繁。在10只动物中的8只中发现了LMI反应性和迟发型超敏反应（DTH）反应性的特异性关联。没有非特异性皮肤试验反应。结果表明，需要连续进行LMI试验以确定反应性趋势，并且这些试验应与其他细胞免疫反应性的体外试验结合使用。
