Assay and the levels of 2-5A-synthetase in lymphocytes of patients with viral, bacterial and autoimmune diseases.

The level of 2-5A-synthetase in extracts of peripheral blood lymphocytes was estimated by the capacity of the enzyme to synthesize 2-5A in the presence of ATP. The 2-5A was then purified on a small column of DEAE-cellulose. Here we show that under or experimental conditions, the amount of 2-5A formed is proportional to the level of the enzyme. The concentration of 2-5A (nmol of AMP/10(6) lymphocytes) in an assay therefore reflects the level of the enzyme. Enhanced levels of 2-5A synthetase were observed in the lymphocytes of patients with viral and bacterial infections. In most of the cases studied, these enhanced levels of the enzyme decreased during the course of infection parallel to recovery. Thus, the level of 2-5A synthetase may indicate the state of the disease and its evolution during the period of treatment; furthermore, it may be a useful marker in monitoring the return to a normal physiological condition. In addition to patients with viral and bacterial infections, enhanced levels of 2-5A synthetase were observed in patients suffering from autoimmune diseases. In five patients studied here, the enhanced levels of 2-5A synthetase remained high at different periods during the course of the disease. These results suggest the presence of circulating interferon throughout the disease.