Wakisaka S, Ichikawa H, Nishimoto T, Matsuo S, Yamamoto K, Nakata T, Akai M
Arch Oral Biol. 1984;29(1):73-5. doi: 10.1016/0003-9969(84)90046-3.
Freshly-extracted human third molars were fixed in Zamboni fixative, demineralized with the mixture of EDTA and the fixative; substance P-like immunoreactivity (SPLI) was revealed by the indirect immunofluorescence technique of Coons. Substance P (SP) was observed in the pulp-dentine zone and the dental pulp. Some of SP-containing fibres ended at the odontoblast layer and did not reach the predentine; others terminated at the predentine surface or penetrated into the predentine. In the predentine, some of SP fibres accompanied odontoblast processes and ended near the mineralized dentine; others changed course transversely at various levels.
新鲜拔除的人类第三磨牙用赞博尼固定液固定，用乙二胺四乙酸（EDTA）和固定液的混合物脱矿；采用孔斯间接免疫荧光技术显示P物质样免疫反应性（SPLI）。在牙髓-牙本质区和牙髓中观察到P物质（SP）。一些含SP的纤维终止于成牙本质细胞层，未到达前期牙本质；另一些则终止于前期牙本质表面或穿入前期牙本质。在前期牙本质中，一些SP纤维伴随成牙本质细胞突起并在矿化牙本质附近终止；另一些则在不同水平横向改变方向。
