Casasco A, Calligaro A, Casasco M, Springall D R, Polak J M, Poggi P, Marchetti C
Institute of Histology and Embryology, University of Pavia, Italy.
Histochemistry. 1990;95(2):115-21. doi: 10.1007/BF00266583.
The peptidergic innervation of human dental pulp was studied with indirect immunofluorescence and immunoperoxidase techniques. Pulpal nerve fibres displaying immunoreactivity for cholecystokinin, calcitonin gene-related peptide, C-terminal flanking peptide of neuropeptide tyrosine, leucine-enkephalin, methionine-enkephalin, neuropeptide K, neuropeptide tyrosine, peptide with N-terminal histidine and C-terminal isoleucine, somatostatin-28, substance P and vasoactive intestinal polypeptide were observed. Immunoreactive axon varicosities were detectable within radicular and coronal nerve trunks and within the nerve plexus of Raschkow in the para-odontoblastic region. Many peptidergic nerve fibres were observed in association with blood vessels of various sizes. Substance P- and calcitonin-gene-related peptide-immunoreactive axons were visible in the odontoblastic layer. The occurrence of VIP- and PHI-immunoreactive fibres lends support to the hypothesis that human tooth may be supplied by parasympathetic nerves. The immunocytochemical results here shown provide a morphological basis to previous experimental studies concerning the possible roles of neuropeptides in nociception mechanisms, control of the blood flow and modulation of the inflammatory response in dental tissues.
采用间接免疫荧光和免疫过氧化物酶技术研究了人牙髓的肽能神经支配。观察到牙髓神经纤维对胆囊收缩素、降钙素基因相关肽、神经肽酪氨酸C末端侧翼肽、亮氨酸脑啡肽、甲硫氨酸脑啡肽、神经肽K、神经肽酪氨酸、N末端组氨酸和C末端异亮氨酸肽、生长抑素-28、P物质和血管活性肠肽呈免疫反应性。在根髓和冠髓神经干以及成牙本质细胞旁区域的Raschkow神经丛内可检测到免疫反应性轴突膨体。观察到许多肽能神经纤维与各种大小的血管相关。在成牙本质细胞层可见P物质和降钙素基因相关肽免疫反应性轴突。血管活性肠肽和胰高血糖素样肽免疫反应性纤维的出现支持了人牙可能由副交感神经支配的假说。此处所示的免疫细胞化学结果为先前关于神经肽在伤害感受机制、血流量控制和牙组织炎症反应调节中可能作用的实验研究提供了形态学基础。
