Young C L, Adamson T C, Vaughan J H, Fox R I
Arthritis Rheum. 1984 Jan;27(1):32-9. doi: 10.1002/art.1780270106.
Synovial membrane biopsy specimens from 15 rheumatoid arthritis patients were examined using routine histologic stains and monoclonal antibodies directed against cell surface antigens. Three patterns of lymphoid cell infiltrates were recognized: 1) diffuse infiltration of T cells that surrounded clusters of germinal center B cells (3 patients); 2) diffuse T cell infiltration, lacking germinal centers (8 patients); and 3) proliferation of subsynovial fibroblasts, with relatively few lymphoid cells (4 patients). The synovial, subsynovial, and perivascular tissues in each of the patterns exhibited a high frequency of HLA-DR antigen, HLA-DS antigen, transferrin receptor, and/or epidermal growth factor receptor. In contrast, normal or osteoarthritic synovial tissues did not display a marked increase of these antigens or receptors. Cells bearing natural killer antigen were infrequent in each of these patterns. Active synovitis, synovial effusions, anemia, and elevated sedimentation rate were present in rheumatoid arthritis patients with each of the three histologic patterns. Immunohistologic characterization of synovial membrane infiltrates by these monoclonal antibodies provides additional information about pathogenesis of rheumatoid arthritis and may help in predicting responses to different therapeutic modalities.
对15例类风湿性关节炎患者的滑膜活检标本进行了检查，采用常规组织学染色和针对细胞表面抗原的单克隆抗体。识别出三种淋巴细胞浸润模式：1）T细胞弥漫性浸润，围绕生发中心B细胞簇（3例患者）；2）弥漫性T细胞浸润，无生发中心（8例患者）；3）滑膜下成纤维细胞增殖，淋巴细胞相对较少（4例患者）。每种模式下的滑膜、滑膜下和血管周围组织均显示出高频率的HLA - DR抗原、HLA - DS抗原、转铁蛋白受体和/或表皮生长因子受体。相比之下，正常或骨关节炎滑膜组织未显示这些抗原或受体的明显增加。在这些模式中，携带自然杀伤抗原的细胞均很少见。三种组织学模式的类风湿性关节炎患者均存在活动性滑膜炎、关节积液、贫血和血沉升高。这些单克隆抗体对滑膜浸润的免疫组织学特征分析为类风湿性关节炎的发病机制提供了额外信息，并可能有助于预测对不同治疗方式的反应。
