Bjornson B H, Pincus S H, DiNapoli A M, Desforges J F
Blood. 1984 Feb;63(2):376-9.
Approximately half of the colony-forming units-culture (CFU-C) from normal peripheral blood are eosinophilic. The purpose of our study was to determine: (1) whether progenitor cells committed to eosinophil or neutrophil maturation would be differentially affected by feedback inhibition, and (2) whether mature eosinophils added to the feeder layers of the culture would inhibit the proliferation of CFU-C in a manner similar to that described for neutrophils. Concentrated eosinophils and neutrophils, obtained by separation on a metrizamide gradient, were added to feeder layers containing either 10(6) autologous whole mononuclear cells (WMNC) or 0.1 ml of leukocyte conditioned media (LCM). The average number of colonies was 123/10(6) nonadherent cells (NAC) cultured. When neutrophils or eosinophils were added to the WMNC feeder layer, the percent inhibition of growth was 40.2% +/- 1.6% (mean +/- SEM) and 42.3% +/- 5.4%, respectively, but the ratio of neutrophil to eosinophil colonies remained constant. No effect was seen when neutrophils or eosinophils were added to an LCM feeder layer. Thus, it appears that the differential control of neutrophil versus eosinophil production in vitro is not regulated through feedback inhibition by mature granulocytes. In addition, these studies suggest that eosinophils, as well as neutrophils, cause inhibition of CFU-C growth when intact cells are the source of colony-stimulating factor (CSF).
正常外周血中约一半的集落形成单位培养物（CFU-C）是嗜酸性粒细胞。我们研究的目的是确定：（1）定向分化为嗜酸性粒细胞或中性粒细胞成熟的祖细胞是否会受到反馈抑制的不同影响，以及（2）添加到培养物饲养层中的成熟嗜酸性粒细胞是否会以类似于中性粒细胞的方式抑制CFU-C的增殖。通过在甲泛葡胺梯度上分离获得的浓缩嗜酸性粒细胞和中性粒细胞，被添加到含有10⁶个自体全单核细胞（WMNC）或0.1 ml白细胞条件培养基（LCM）的饲养层中。培养的10⁶个非贴壁细胞（NAC）的平均集落数为123个。当中性粒细胞或嗜酸性粒细胞添加到WMNC饲养层时，生长抑制百分比分别为40.2%±1.6%（平均值±标准误）和42.3%±5.4%，但中性粒细胞集落与嗜酸性粒细胞集落的比例保持恒定。当中性粒细胞或嗜酸性粒细胞添加到LCM饲养层时未观察到影响。因此，体外中性粒细胞与嗜酸性粒细胞生成的差异控制似乎不是通过成熟粒细胞的反馈抑制来调节的。此外，这些研究表明，当完整细胞是集落刺激因子（CSF）的来源时，嗜酸性粒细胞以及中性粒细胞会导致CFU-C生长受到抑制。
