Savard P, Mérand Y, Dupont A
Brain Res. 1984 Feb 6;292(2):349-55. doi: 10.1016/0006-8993(84)90770-4.
Neonatal hypothyroidism is known to induce the accumulation of substance P and serotonin in many discrete brain nuclei of the rat. In this work, we have studied the effects of p-chlorophenylalanine (PCPA), a serotonin (5-HT) depleting drug, on the regional distribution of substance P in the brains of neonatally-induced hypothyroid and euthyroid rats. PCPA injections were given 48 and 24 h before sacrifice and the peptide content of discrete brain nuclei, removed by punches of frozen brain slices, was measured by radioimmunoassay. In hypothyroid animals, substance P levels were significantly increased in the n. paraventricularis, n. medialis thalami, n. arcuatus. n. mamillaris medialis, substantia nigra, area ventralis tegmenti, n. interpeduncularis, substantia grisea and n. dorsalis raphes. This hypothyroid-induced accumulation of substance P was completely abolished, and even reversed in some nuclei, by PCPA treatment. Interestingly, in normal rats, following PCPA treatment, the substance P level was increased in n. paraventricularis pars magnocellularis, n. ventromedialis (hypothalami), n. dorsomedialis pars dorsalis (hypothalami), n. amygdaloideus centralis, area ventralis tegmenti, substantia grisea, n. medianus raphes and n. dorsalis raphes. These results lend support to the existence of a physiological relationship between both substance P and serotonergic systems.
已知新生儿甲状腺功能减退会导致大鼠许多离散脑核中P物质和5-羟色胺的积累。在本研究中，我们研究了5-羟色胺耗竭药物对氯苯丙氨酸（PCPA）对新生期诱导的甲状腺功能减退和甲状腺功能正常大鼠脑内P物质区域分布的影响。在处死前48小时和24小时注射PCPA，通过冷冻脑片打孔取出离散脑核的肽含量，采用放射免疫分析法进行测定。在甲状腺功能减退的动物中，室旁核、丘脑内侧核、弓状核、内侧乳头体核、黑质、腹侧被盖区、脚间核、灰质和中缝背核中的P物质水平显著升高。PCPA处理完全消除了甲状腺功能减退诱导的P物质积累，甚至在某些核中使其逆转。有趣的是，在正常大鼠中，PCPA处理后，室旁核大细胞部、腹内侧核（下丘脑）、背内侧核背侧部（下丘脑）、杏仁中央核、腹侧被盖区、灰质、中缝正中核和中缝背核中的P物质水平升高。这些结果支持了P物质和5-羟色胺能系统之间存在生理关系的观点。
