Olschowka J A, O'Donohue T L, Mueller G P, Jacobowitz D M
Peptides. 1982 Nov-Dec;3(6):995-1015. doi: 10.1016/0196-9781(82)90071-7.
Using the indirect immunofluorescent technique, corticotropin releasing factor (CRF)-like immunoreactive nerve fibers and cell bodies were observed to be widely distributed in rat brain. A detailed stereotaxic atlas of CRF-like immunoreactive neurons was prepared. Large numbers of CRF-containing perikarya were observed in the nucleus paraventricularis, with scattered cells in the following nuclei: accumbens, interstitialis stria terminalis, preopticus medialis, supraopticus, periventricularis hypothalami, amygdaloideus centralis, dorsomedialis, substantia grisea centralis, parabrachialis dorsalis and ventralis, tegmenti dorsalis lateralis, vestibularis medialis, tractus solitarius and reticularis lateralis. The most intense staining of CRF-containing fibers was observed in the external lamina of the median eminence. Moderate numbers of CRF-like fibers were observed in the following nuclei: lateralis and medialis septi, tractus diagonalis, interstitialis stria terminalis, preopticus medialis, supraopticus, periventricularis thalami and hypothalami, paraventricularis, anterior ventralis and medialis thalami, rhomboideus, amygdaloideus centralis, habenulae lateralis, dorsomedialis, ventromedialis, substantia grisea centralis, cuneiformis, parabrachialis dorsalis and ventralis, tegmenti dorsalis lateralis, cerebellum, vestibularis medialis, reticularis lateralis, substantia gelatinosa trigemini and lamina I and II of the dorsal horn of the spinal cord. The present findings suggest that a CRF-like peptide may be involved in a neurotransmitter or neuromodulator role, as well as a hypophysiotropic role.
采用间接免疫荧光技术，观察到促肾上腺皮质激素释放因子（CRF）样免疫反应性神经纤维和细胞体广泛分布于大鼠脑内。制备了CRF样免疫反应性神经元的详细立体定位图谱。在室旁核中观察到大量含CRF的核周体，在以下核中有散在细胞：伏隔核、终纹床核间质、内侧视前核、视上核、下丘脑室周核、中央杏仁核、背内侧核、中央灰质、臂旁背核和腹核、外侧背侧被盖、内侧前庭核、孤束核和外侧网状核。在正中隆起的外层观察到含CRF纤维的染色最强。在以下核中观察到中等数量的CRF样纤维：外侧和内侧隔核、斜角带、终纹床核间质、内侧视前核、视上核、丘脑和下丘脑室周核、室旁核、丘脑前腹侧和内侧核、菱形核、中央杏仁核、外侧缰核、背内侧核、腹内侧核、中央灰质、楔状核、臂旁背核和腹核、外侧背侧被盖、小脑、内侧前庭核、外侧网状核、三叉神经胶状质以及脊髓背角的I层和II层。目前的研究结果表明，一种CRF样肽可能参与神经递质或神经调质作用以及促垂体作用。
