Short P E, Williams C E, Enayat M S, Picken A M, Hill F G
J Clin Pathol. 1984 Feb;37(2):194-9. doi: 10.1136/jcp.37.2.194.
Factor VIII related antigen has been measured and epitope distribution has been explored by testing the degree of parallelism between standard and test plasma dose response curves using an enzyme immunoassay. Normal plasma, plasma fractions, and plasma from patients with haemophilia and von Willebrand's disease have been tested. All showed parallelism except for plasma from patients with the variant type IIA von Willebrand's disease, of which 10 had parallel and five had non-parallel dose response curves when compared with that of normal plasma. In one family plasma from seven members showed parallelism but from four others did not. An unrelated patient was tested on three occasions, and although the samples were parallel to each other, no sample was parallel to the standard. No correlation was found between parallelism as shown by the enzyme immunoassay and differences in factor VIII related antigen multimeric pattern, including triplet configuration, seen in the type IIA patients.
采用酶免疫测定法，通过检测标准血浆与测试血浆剂量反应曲线之间的平行程度，对VIII因子相关抗原进行了测定，并探究了表位分布情况。对正常血浆、血浆组分以及血友病和血管性血友病患者的血浆进行了检测。除IIA型血管性血友病变异型患者的血浆外，其他所有样本均显示出平行性。与正常血浆相比，10例IIA型血管性血友病变异型患者的血浆剂量反应曲线呈平行，5例呈非平行。在一个家族中，7名成员的血浆显示出平行性，但另外4名成员的血浆则不然。对一名无亲缘关系的患者进行了三次检测，尽管样本之间相互平行，但没有一个样本与标准血浆平行。在IIA型患者中，酶免疫测定显示的平行性与VIII因子相关抗原多聚体模式（包括三联体构型）的差异之间未发现相关性。
