在持续感染期间，通过单克隆抗体可检测到的HVJ（仙台病毒）HN糖蛋白的抗原变异。

Antigenic variation of HVJ (Sendai virus) HN glycoprotein detectable by monoclonal antibodies during persistent infection.

作者信息

Sato H, Ogura H, Tanaka J, Hatano M

出版信息

J Gen Virol. 1984 Jan;65 ( Pt 1):185-9. doi: 10.1099/0022-1317-65-1-185.

PMID:6198445

Abstract

Three newly established monoclonal hybridoma antibodies to the haemagglutinin molecule of HVJ, designated A7, B3 and F11, recognize operationally non-overlapping antigenic determinants and have neutralizing activity. Using these antibodies, the frequencies of occurrence of neutralization-resistant antigenic variants were analysed in virus populations released from four cell lines persistently infected with HVJ, namely GM2-HVJ, LLCMK2-HVJ, Vero-HVJ and GEsl-HVJ at various passage stages. Antigenic variants were selected from culture fluids of these HVJ carrier cells at a total frequency of 10(-3.3), 10(-3.8) and 10(-3.6) by monoclonal antibodies A7, B3 and F11, respectively. These values were considerably higher than those of 10(-4.7) to 10(-5.2) detected in a stock preparation of wild-type virus with these antibodies. All the variant viruses isolated as above were negative in neutralization, haemagglutination inhibition and immunofluorescent staining tests with each monoclonal antibody used for their isolation, but were positive with the other antibodies.

摘要

三种新建立的针对仙台病毒（HVJ）血凝素分子的单克隆杂交瘤抗体，分别命名为A7、B3和F11，识别在操作上不重叠的抗原决定簇并具有中和活性。利用这些抗体，分析了从四个持续感染HVJ的细胞系（即GM2-HVJ、LLCMK2-HVJ、Vero-HVJ和GEsl-HVJ）在不同传代阶段释放的病毒群体中抗中和抗原变体的出现频率。分别用单克隆抗体A7、B3和F11从这些HVJ载体细胞的培养液中筛选出抗原变体，总频率分别为10^(-3.3)、10^(-3.8)和10^(-3.6)。这些值显著高于用这些抗体在野生型病毒储备液中检测到的10^(-4.7)至10^(-5.2)。上述分离出的所有变异病毒在用用于分离它们的每种单克隆抗体进行的中和、血凝抑制和免疫荧光染色试验中均为阴性，但用其他抗体检测时为阳性。