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利用单克隆抗体揭示的1型人副流感病毒分离株的抗原多样性及其与仙台病毒的免疫学关系。

Antigenic diversity of human parainfluenza virus type 1 isolates and their immunological relationship with Sendai virus revealed by using monoclonal antibodies.

作者信息

Komada H, Kusagawa S, Orvell C, Tsurudome M, Nishio M, Bando H, Kawano M, Matsumura H, Norrby E, Ito Y

机构信息

Department of Microbiology, Mie University School of Medicine, Japan.

出版信息

J Gen Virol. 1992 Apr;73 ( Pt 4):875-84. doi: 10.1099/0022-1317-73-4-875.

Abstract

Fifty-six monoclonal antibodies (MAbs) directed against human parainfluenza virus type 1 (hPIV-1) were prepared in order to identify the structural proteins of hPIV-1, to examine the immunological relationship between hPIV-1 and Sendai virus (SV), and to determine the antigenic diversity of clinical isolates of hPIV-1. In addition, 41 MAbs characterized previously and directed against SV were used for immunological comparison of SV and hPIV-1 isolates. Of the MAbs against hPIV-1, two reacted with phospho (P) protein, 11 with nucleocapsid protein (NP), 24 with haemagglutinin-neuraminidase (HN) protein and 19 with fusion (F) protein. With the aid of MAbs against hPIV-1 and those against SV showing cross-reactivity with hPIV-1, the structural proteins of hPIV-1 were identified; p83, p56, p34, gp74 and gp60 of hPIV-1 were identified as the P, NP, M, HN and F proteins, respectively. The MAbs against the P protein and NP of hPIV-1 showed limited cross-reactivity with SV, whereas they had high reactivity with clinical isolates of hPIV-1. Interestingly, one MAb against the NP of hPIV-1 lacked reactivity with clinical isolates which were isolated in the 1970s and 1980s. The MAbs against the HN of hPIV-1 also exhibited quite limited reactivity with SV and the clinical isolates; two groups of HN-specific MAbs showed almost no reactivity with the clinical isolates from the 1970s and 1980s, similarly to the NP-specific MAb. However, anti-HN MAbs belonging to the two groups showing specific activities (neuraminidase inhibition and haemolysis inhibition) reacted with almost all clinical isolates. On the other hand, although anti-F protein MAbs had limited reactivity with SV, they showed reactivity with almost all hPIV-1 isolates. The MAbs against the P, NP, M, HN and F proteins of SV also showed limited cross-reactivity with the clinical hPIV-1 isolates, and this reactivity was independent of the time and place of isolation, except for that of the F protein. These results confirm that although hPIV-1 is related to SV, it is antigenically distinct from it.

摘要

制备了56种针对1型人副流感病毒(hPIV-1)的单克隆抗体(MAb),以鉴定hPIV-1的结构蛋白,研究hPIV-1与仙台病毒(SV)之间的免疫关系,并确定hPIV-1临床分离株的抗原多样性。此外,还使用了41种先前鉴定的针对SV的MAb对SV和hPIV-1分离株进行免疫比较。在针对hPIV-1的MAb中,两种与磷酸化(P)蛋白反应,11种与核衣壳蛋白(NP)反应,24种与血凝素神经氨酸酶(HN)蛋白反应,19种与融合(F)蛋白反应。借助针对hPIV-1的MAb以及与hPIV-1表现出交叉反应的针对SV的MAb,鉴定出了hPIV-1的结构蛋白;hPIV-1的p83、p56、p34、gp74和gp60分别被鉴定为P、NP、M、HN和F蛋白。针对hPIV-1的P蛋白和NP的MAb与SV表现出有限的交叉反应,而它们与hPIV-1临床分离株具有高反应性。有趣的是,一种针对hPIV-1的NP的MAb与20世纪70年代和80年代分离的临床分离株没有反应性。针对hPIV-1的HN的MAb与SV和临床分离株也表现出相当有限的反应性;两组HN特异性MAb与20世纪70年代和80年代的临床分离株几乎没有反应性,与NP特异性MAb类似。然而,属于两组具有特定活性(神经氨酸酶抑制和溶血抑制)的抗HN MAb与几乎所有临床分离株反应。另一方面,尽管抗F蛋白MAb与SV的反应性有限,但它们与几乎所有hPIV-1分离株都有反应性。针对SV的P、NP、M、HN和F蛋白的MAb与临床hPIV-1分离株也表现出有限的交叉反应,除了F蛋白外,这种反应性与分离的时间和地点无关。这些结果证实,尽管hPIV-1与SV有关,但在抗原性上与它不同。

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