Komada H, Kusagawa S, Orvell C, Tsurudome M, Nishio M, Bando H, Kawano M, Matsumura H, Norrby E, Ito Y
Department of Microbiology, Mie University School of Medicine, Japan.
J Gen Virol. 1992 Apr;73 ( Pt 4):875-84. doi: 10.1099/0022-1317-73-4-875.
Fifty-six monoclonal antibodies (MAbs) directed against human parainfluenza virus type 1 (hPIV-1) were prepared in order to identify the structural proteins of hPIV-1, to examine the immunological relationship between hPIV-1 and Sendai virus (SV), and to determine the antigenic diversity of clinical isolates of hPIV-1. In addition, 41 MAbs characterized previously and directed against SV were used for immunological comparison of SV and hPIV-1 isolates. Of the MAbs against hPIV-1, two reacted with phospho (P) protein, 11 with nucleocapsid protein (NP), 24 with haemagglutinin-neuraminidase (HN) protein and 19 with fusion (F) protein. With the aid of MAbs against hPIV-1 and those against SV showing cross-reactivity with hPIV-1, the structural proteins of hPIV-1 were identified; p83, p56, p34, gp74 and gp60 of hPIV-1 were identified as the P, NP, M, HN and F proteins, respectively. The MAbs against the P protein and NP of hPIV-1 showed limited cross-reactivity with SV, whereas they had high reactivity with clinical isolates of hPIV-1. Interestingly, one MAb against the NP of hPIV-1 lacked reactivity with clinical isolates which were isolated in the 1970s and 1980s. The MAbs against the HN of hPIV-1 also exhibited quite limited reactivity with SV and the clinical isolates; two groups of HN-specific MAbs showed almost no reactivity with the clinical isolates from the 1970s and 1980s, similarly to the NP-specific MAb. However, anti-HN MAbs belonging to the two groups showing specific activities (neuraminidase inhibition and haemolysis inhibition) reacted with almost all clinical isolates. On the other hand, although anti-F protein MAbs had limited reactivity with SV, they showed reactivity with almost all hPIV-1 isolates. The MAbs against the P, NP, M, HN and F proteins of SV also showed limited cross-reactivity with the clinical hPIV-1 isolates, and this reactivity was independent of the time and place of isolation, except for that of the F protein. These results confirm that although hPIV-1 is related to SV, it is antigenically distinct from it.
制备了56种针对1型人副流感病毒(hPIV-1)的单克隆抗体(MAb),以鉴定hPIV-1的结构蛋白,研究hPIV-1与仙台病毒(SV)之间的免疫关系,并确定hPIV-1临床分离株的抗原多样性。此外,还使用了41种先前鉴定的针对SV的MAb对SV和hPIV-1分离株进行免疫比较。在针对hPIV-1的MAb中,两种与磷酸化(P)蛋白反应,11种与核衣壳蛋白(NP)反应,24种与血凝素神经氨酸酶(HN)蛋白反应,19种与融合(F)蛋白反应。借助针对hPIV-1的MAb以及与hPIV-1表现出交叉反应的针对SV的MAb,鉴定出了hPIV-1的结构蛋白;hPIV-1的p83、p56、p34、gp74和gp60分别被鉴定为P、NP、M、HN和F蛋白。针对hPIV-1的P蛋白和NP的MAb与SV表现出有限的交叉反应,而它们与hPIV-1临床分离株具有高反应性。有趣的是,一种针对hPIV-1的NP的MAb与20世纪70年代和80年代分离的临床分离株没有反应性。针对hPIV-1的HN的MAb与SV和临床分离株也表现出相当有限的反应性;两组HN特异性MAb与20世纪70年代和80年代的临床分离株几乎没有反应性,与NP特异性MAb类似。然而,属于两组具有特定活性(神经氨酸酶抑制和溶血抑制)的抗HN MAb与几乎所有临床分离株反应。另一方面,尽管抗F蛋白MAb与SV的反应性有限,但它们与几乎所有hPIV-1分离株都有反应性。针对SV的P、NP、M、HN和F蛋白的MAb与临床hPIV-1分离株也表现出有限的交叉反应,除了F蛋白外,这种反应性与分离的时间和地点无关。这些结果证实,尽管hPIV-1与SV有关,但在抗原性上与它不同。