急性胰腺炎的诊断：脂肪酶ELISA检测法提高了敏感性。

The diagnosis of acute pancreatitis: enhanced sensitivity from an ELISA assay of lipase.

作者信息

Bowen M, Cooper E H, McMahon M J

出版信息

Biomed Pharmacother. 1983;37(8):395-8.

PMID:6199049

Abstract

An enzyme linked immunosorbent assay (ELISA) for human pancreatic lipase was compared, for diagnostic sensitivity, to a chromogenic assay of total plasma amylase in 37 patients with acute pancreatitis. Data from patients were related to a control group of 38 patients with gastrointestinal disease but without pancreatic diseases. During the first 24 hours of admission, lipase was elevated 131 standard deviations (SD) above the mean of the control range compared to 25 SD for amylase (p less than 0.001). When amylase fell to within the control range (less than 531 iu/l) lipase was still elevated at a mean of 11 SD above the control mean. In patients followed for longer, lipase usually remained above normal for at least ten days, whereas amylase lay within 1 SD of the mean of the control samples in most plasma samples after 4 days. These data show that lipase is considerably more sensitive than total amylase for the diagnosis of acute pancreatitis.

摘要

在37例急性胰腺炎患者中，对一种用于检测人胰脂肪酶的酶联免疫吸附测定（ELISA）与总血浆淀粉酶的显色测定的诊断敏感性进行了比较。患者数据与38例患有胃肠道疾病但无胰腺疾病的对照组相关。在入院后的头24小时内，脂肪酶升高至高于对照范围平均值131个标准差（SD），而淀粉酶为25个SD（p<0.001）。当淀粉酶降至对照范围内（低于531 iu/l）时，脂肪酶仍高于对照平均值11个SD。在随访时间更长的患者中，脂肪酶通常至少十天保持高于正常水平，而在4天后的大多数血浆样本中，淀粉酶处于对照样本平均值的1个SD范围内。这些数据表明，脂肪酶在诊断急性胰腺炎方面比总淀粉酶敏感得多。