Trinchieri G, Matsumoto-Kobayashi M, Clark S C, Seehra J, London L, Perussia B
J Exp Med. 1984 Oct 1;160(4):1147-69. doi: 10.1084/jem.160.4.1147.
The present study shows that recombinant interleukin 2 (IL-2) purified to homogeneity induces a rapid and potent enhancement of spontaneous cytotoxicity of human peripheral blood lymphocytes. The cells mediating cytotoxicity after 18-h treatment with IL-2 have surface markers of natural killer (NK) cells and are generated from the peripheral blood subset containing spontaneous cytotoxic cells. A parallel production of gamma interferon (IFN-gamma) is induced by recombinant IL-2 (rIL-2), and NK cells appear to be the major producer cells, whereas T cells are unable to produce IFN-gamma under these experimental conditions. However, the kinetics of the enhancement of cytotoxicity are faster than those of IFN-gamma production, and monoclonal anti-IFN-gamma antibodies do not suppress this effect, making it unlikely that the IFN-gamma produced is responsible for the enhancement. The enhancement of NK cell activity induced by rIL-2 precedes any proliferative response of the lymphocytes, which is instead observed in longer-term cultures of both NK and T cells.
本研究表明，纯化至同质的重组白细胞介素2（IL-2）可迅速且显著增强人外周血淋巴细胞的自发细胞毒性。用IL-2处理18小时后介导细胞毒性的细胞具有自然杀伤（NK）细胞的表面标志物，并且源自含有自发细胞毒性细胞的外周血亚群。重组IL-2（rIL-2）可诱导γ干扰素（IFN-γ）的平行产生，NK细胞似乎是主要的产生细胞，而在这些实验条件下T细胞无法产生IFN-γ。然而，细胞毒性增强的动力学比IFN-γ产生的动力学更快，并且单克隆抗IFN-γ抗体不会抑制这种效应，这使得所产生的IFN-γ不太可能是导致增强的原因。rIL-2诱导的NK细胞活性增强先于淋巴细胞的任何增殖反应，而在NK细胞和T细胞的长期培养中均观察到增殖反应。
