Lavrijsen K, Verwilghen R L
Hemoglobin. 1983;7(2):159-79. doi: 10.3109/03630268309048644.
We investigated hemoglobin synthesis in suspension cultures of liver erythroid cells obtained from calf fetuses of 103 to 198 days. A significant amount of radioactivity was associated with adult hemoglobins which were separated from the fetal hemoglobins by isoelectric focusing, even after purification of the cell hemolysates by chromatography on Sephadex G-100. A radioactive beta-globin fraction was isolated by chromatography on carboxymethyl-cellulose from hemolysates, which were first fractionated on Sephadex G-100. Fingerprint analysis of peptides obtained by trypsinolysis of radioactive beta-globin chains revealed that its structure was closely related to that of beta-globin, isolated from cow bone marrow cells. The amount of beta-globin which was synthesized by calf liver cells varied from 0.3 to 3.5% of the non-alpha globin chains and remained at a low level for all the fetuses which were studied. Our results indicate that the bovine fetal liver is a valuable model to investigate the switch from fetal to adult hemoglobin synthesis.
我们研究了从103至198天胎龄的小牛胎儿获取的肝脏红系细胞悬浮培养物中的血红蛋白合成情况。即使通过Sephadex G - 100柱层析对细胞溶血产物进行纯化后，通过等电聚焦从胎儿血红蛋白中分离出的成人血红蛋白仍带有大量放射性。首先在Sephadex G - 100上进行分级分离，然后通过羧甲基纤维素柱层析从溶血产物中分离出一个放射性β - 珠蛋白组分。对放射性β - 珠蛋白链经胰蛋白酶消化得到的肽段进行指纹分析表明，其结构与从牛骨髓细胞中分离出的β - 珠蛋白结构密切相关。小牛肝细胞合成的β - 珠蛋白量占非α珠蛋白链的0.3%至3.5%，并且在所研究的所有胎儿中均维持在较低水平。我们的结果表明，牛胎儿肝脏是研究从胎儿血红蛋白合成向成人血红蛋白合成转变的一个有价值的模型。
